University of Southern California Dissertations and Theses

Kaposi's sarcoma‐associated herpesvirus‐Bcl-2 mediated regulation of NM23-H2 is required for KSHV lytic replication

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Kaposi's sarcoma‐associated herpesvirus‐Bcl-2 mediated regulation of NM23-H2 is required for KSHV lytic replication

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Abstract (if available)

Abstract All viruses need to interact with cellular complexes to escape host immune response and keep their infection persistent and tumorigenic. Kaposi's sarcoma‐associated herpesvirus (KSHV) encodes a viral Bcl-2 protein (kBcl2) critical for KSHV immune evasion of host defense. Up to now, two distinct functions—anti‐apoptosis and anti‐autophagy—have been identified of kBcl2 in viral lifecycle. A recent study showed that kBcl-2 is critically required for the lytic replication of KSHV, which is surprisingly independent of kBcl-2's anti‐apoptosis and anti‐autophagy activities. Moreover, residue E14 of kBcl-2 has been shown to be essential for viral replication in that mutation of residue E14 to alanine (E14A) abolishes the ability of kBcl-2 in promoting viral replication. However, the molecular mechanism by which kBcl-2 mediates viral progeny production remains unknown. Our recent study showed that NM23-H2 interacts with kBcl-2 and this interaction is negotiated by the E14 residue of kBcl-2. Moreover, kBcl-2 colocalizes with NM23-H2 at the ER, and a depletion of NM23-H2 impairs viral lytic replication. Thus, we postulate that kBcl-2 regulation of host NM23-H2 represents a significant new mechanism required for viral lytic replication.

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Asset Metadata

Creator Zhao, Shihui (author)

Core Title Kaposi's sarcoma‐associated herpesvirus‐Bcl-2 mediated regulation of NM23-H2 is required for KSHV lytic replication

School Keck School of Medicine

Degree Master of Science

Degree Program Molecular Microbiology and Immunology

Publication Date 04/11/2018

Defense Date 03/15/2018

Publisher University of Southern California (original), University of Southern California. Libraries (digital)

Tag Kaposi sarcoma‐associated herpesvirus,kBcl-2,lytic replication,NM23-H2,OAI-PMH Harvest

Language English

Contributor Electronically uploaded by the author (provenance)

Advisor Liang, Chengyu (committee member)

Creator Email shihuiz@usc.edu

Permanent Link (DOI) https://doi.org/10.25549/usctheses-c89-12429

Unique identifier UC11669022

Identifier etd-ZhaoShihui-6183.pdf (filename),usctheses-c89-12429 (legacy record id)

Legacy Identifier etd-ZhaoShihui-6183.pdf

Dmrecord 12429

Document Type Thesis

Rights Zhao, Shihui

Type texts

Source University of Southern California (contributing entity), University of Southern California Dissertations and Theses (collection)

Access Conditions The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the a...

Repository Name University of Southern California Digital Library

Repository Location USC Digital Library, University of Southern California, University Park Campus MC 2810, 3434 South Grand Avenue, 2nd Floor, Los Angeles, California 90089-2810, USA