Advanced applications of high frequency single beam acoustic tweezers in fluid and cell mechanics

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Abstract (if available)

Abstract Single beam acoustic (SBA) tweezers represent a new technology for particle and cell trapping. The advantages of SBA tweezers are their deep penetration into tissues, reduction of tissue damage, and ease of application to in vivo study. The use of these tools for applications in drug delivery in vivo has to meet the following conditions: large penetration depth, strong trapping force and tissue safety. The reasonable penetration depth of SBA tweezers for developing in vivo applications was discovered by a previous study conducted in water with zero velocity. However, capturing objects in a flowing fluid can provide more meaningful results. In the dissertation, we investigated the capability of SBA tweezers to trap red blood cells (RBCs) and polystyrene microparticles in flowing RBC suspensions. ❧ On the other hand, cellular biomechanics represent a significant characteristic in metastasis. It has already been known that highly invasive cancer cells are typically more compliant than weakly invasive cancer cells, allowing them to migrate easily. The presence of chemotherapy-resistant cells can be detected in the bone marrow (BM) and peripheral blood (PB) and is called minimal residual disease (MRD). Once MRD is diagnosed, patients are associated with a higher risk of relapse in acute lymphoblastic leukemia (ALL). Studying cell mechanics for ALL in drug resistance is crucial. However, the research of the mechanical properties in drug resisted ALL cells has not been widely discovered. Single beam acoustic (SBA) tweezers are a novel and promising technology to quantify the mechanical phenotype of cells. Here, a non-contact acoustic trapping method based on the SBA tweezer for quantification of deformability of numbers of types ALL cells and those survived ALL cells (drug resisted ALL) treated by chemotherapy drugs was demonstrated. The research showed that those survived cells after 7-day chemotherapy drug treatment revealed more compliant than ALL cells in media control by using the SBA tweezer. Two antibodies and oleic acid were also added to cells to investigate the mechanical property, and reveled a larger compliance comparing with control group. The study demonstrated that SBA tweezers is a promising label free tool for non-contact quantitative discrimination of mechanical phenotypes of cells. ❧ Young’s modulus plays an important role in cell mechanics due to a fundamental feature in regulating a diverse array of physiological processes. Traditional methods, AFM, micropipette, optical tweezers and microfluidic channels, have their own disadvantages. A new concept, called Young’s modulus map, was proposed to estimate leukemia cells by SBA tweezers associated with microparticles with different agarose concentrations. In addition, finite element analysis (FEA) in FEBio software was used to simulate the deformation of cells. ❧ The motility of ALL in the media control and drug resisted ALL cells was studied. The experimental results revealed that the drug resisted ALL cells after the treatment decreased the velocity and migration distance than cells in media control. These results elaborated that the stiffness of drug resisted ALL cells expressed the inverse relationship with the motility and migration pattern of leukemia cells proved by using deep-tissue imaging.

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University of Southern California Dissertations and Theses

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Asset Metadata

Creator Liu, Hsiao-Chuan (author)

Core Title Advanced applications of high frequency single beam acoustic tweezers in fluid and cell mechanics

School Viterbi School of Engineering

Degree Doctor of Philosophy

Degree Program Biomedical Engineering

Publication Date 02/20/2019

Defense Date 01/11/2019

Publisher University of Southern California (original), University of Southern California. Libraries (digital)

Tag acute lymphoblastic leukemia,agarose microparticles,cell mechanics,cell motility,deformability,drug resistance,finite element analysis,minimal residual disease,OAI-PMH Harvest,single beam acoustic tweezers,Young's modulus

Format application/pdf (imt)

Language English

Contributor Electronically uploaded by the author (provenance)

Advisor Kim, Yong-Mi (committee chair), Shung, K. Kirk (committee chair), Zhou, Qifa (committee member)

Creator Email hcliu0615@gmail.com,hsiaochu@usc.edu

Permanent Link (DOI) https://doi.org/10.25549/usctheses-c89-124557

Unique identifier UC11675854

Identifier etd-LiuHsiaoCh-7092.pdf (filename),usctheses-c89-124557 (legacy record id)

Legacy Identifier etd-LiuHsiaoCh-7092.pdf

Dmrecord 124557

Document Type Dissertation

Format application/pdf (imt)

Rights Liu, Hsiao-Chuan

Type texts

Source University of Southern California (contributing entity), University of Southern California Dissertations and Theses (collection)

Access Conditions The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the a...

Repository Name University of Southern California Digital Library

Repository Location USC Digital Library, University of Southern California, University Park Campus MC 2810, 3434 South Grand Avenue, 2nd Floor, Los Angeles, California 90089-2810, USA