Shewanella spc. 16S rDNA signal attenuation due to UVC, gamma and cryogenic lab conditions

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Abstract (if available)

Abstract In understanding DNA as a biomarker over geologic time scales, it is important to determine the longevity of the signal under common environmental stress. Shewanella spp of bacteria in aqueous solution were subjucted to lethal doses of UVC radiation, gamma radiation and thermal and cryogenic conditions. The species succumbed to UVC quickly with a post-mortem DNA signal at least 2 orders of magnitude longer than the life of the cell. Cryogenic stress revealed differential death, but no obvious signal degradation and gamma radiation showed some degradation with cell death but more data are needed to determine a half life for the signal. This study indicates a DNA signal could last 0 (thermal conditions) to 4 or more (cryogenic conditions) orders of magnitude beyond cell death depending on the environment and the time of cell death.

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University of Southern California Dissertations and Theses

Asset Metadata

Creator Fouch, Brenda L. (author)

Core Title Shewanella spc. 16S rDNA signal attenuation due to UVC, gamma and cryogenic lab conditions

School College of Letters, Arts and Sciences

Degree Master of Science

Degree Program Geological Sciences

Publication Date 04/18/2008

Defense Date 04/09/2008

Publisher University of Southern California (original), University of Southern California. Libraries (digital)

Tag biomarker,cyrogenic,DNA signal longevity,gamma,OAI-PMH Harvest,rock record,UVC

Language English

Advisor Nealson, Kenneth H. (committee chair), Bottjer, David J. (committee member), Corsetti, Frank A. (committee member)

Creator Email brenda.fouch@us.army.mil

Permanent Link (DOI) https://doi.org/10.25549/usctheses-m1151

Unique identifier UC195465

Identifier etd-Fouch-20080418 (filename),usctheses-m40 (legacy collection record id),usctheses-c127-60237 (legacy record id),usctheses-m1151 (legacy record id)

Legacy Identifier etd-Fouch-20080418.pdf

Dmrecord 60237

Document Type Thesis

Rights Fouch, Brenda L.

Type texts

Source University of Southern California (contributing entity), University of Southern California Dissertations and Theses (collection)

Repository Name Libraries, University of Southern California

Repository Location Los Angeles, California

Repository Email cisadmin@lib.usc.edu