University of Southern California Dissertations and Theses

Biochemical modifications and regulation of the gastric H,K-ATPase

(USC Thesis Other)

Biochemical modifications and regulation of the gastric H,K-ATPase

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Abstract (if available)

Abstract The α/β heterodimeric gastric H,K-ATPase is the enzyme responsible for gastric HCl secretion by the parietal cell. It is an integral membrane protein of the extensive system of intracellular membranes known as tubulovesicles of resting parietal cells, and it is trafficked to the apical plasma membrane upon stimulation by a regulated fusion of tubulovesicles with the apical membrane. There are, however, further mechanisms that may regulate HCl secretion, and these will be explored in this doctoral dissertation. Very little is known about the direct regulation of the H,K-ATPase and the mechanisms that regulate the enzymatic activity of the enzyme. Furthermore, little has been reported about certain posttranslational modifications that may occur on the H,K-ATPase, specifically ubiquitination and glutathiolation, which may have implications in the regulation of the activity of the H,K-ATPase within the parietal cell. The regulation of membrane recycling, particularly the tight packing of tubulovesicles in a resting parietal cell is also poorly understood.

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Asset Metadata

Creator Nagatani, Gerald Kenji (author)

Core Title Biochemical modifications and regulation of the gastric H,K-ATPase

School School of Pharmacy

Degree Doctor of Philosophy

Degree Program Pharmaceutical Sciences

Publication Date 05/04/2009

Defense Date 01/29/2009

Publisher University of Southern California (original), University of Southern California. Libraries (digital)

Tag glutathiolation,glutathione,H,K-ATPase,OAI-PMH Harvest,parietal cell,phosphoinositides,proton pump,tubulovesicles,ubiquitin,ubiquitination

Language English

Contributor Electronically uploaded by the author (provenance)

Advisor Okamoto, Curtis T. (committee chair), Farley, Robert A. (committee member), Haworth, Ian S. (committee member)

Creator Email nagatani@gmail.com,nagatani@usc.edu

Permanent Link (DOI) https://doi.org/10.25549/usctheses-m2169

Unique identifier UC1424794

Identifier etd-Nagatani-2671 (filename),usctheses-m40 (legacy collection record id),usctheses-c127-224408 (legacy record id),usctheses-m2169 (legacy record id)

Legacy Identifier etd-Nagatani-2671.pdf

Dmrecord 224408

Document Type Dissertation

Rights Nagatani, Gerald Kenji

Type texts

Source University of Southern California (contributing entity), University of Southern California Dissertations and Theses (collection)

Repository Name Libraries, University of Southern California

Repository Location Los Angeles, California

Repository Email cisadmin@lib.usc.edu