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CONTROLLING MEMBRANE PROTEIN FOLDING USING PHOTORESPONSIVE SURFACTANT
by
Chia Hao Chang
A Dissertation Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
DOCTOR OF PHILOSOPHY
(CHEMICAL ENGINEERING)
August 2012
Copyright 2012 Chia Hao Chang
Object Description
| Title | Controlling membrane protein folding using photoresponsive surfactant |
| Author | Chang, Chia Hao |
| Author email | chang7@usc.edu;coucal.tw@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Chemical Engineering |
| School | Viterbi School of Engineering |
| Date defended/completed | 2012-06-11 |
| Date submitted | 2012-07-09 |
| Date approved | 2012-07-10 |
| Restricted until | 2012-07-10 |
| Date published | 2012-07-10 |
| Advisor (committee chair) | Lee, C. Ted, Jr. |
| Advisor (committee member) |
Shing, Katherine Langen, Ralf |
| Abstract | Membrane proteins perform a number of roles in biological function. Membrane lipids can self assembly into numerous different phases in aqueous solution, including micelles, vesicles and lamellar phases. However, the phase properties of biological membranes are far more complex. Many membrane proteins require specific lipids to be present in the membrane to be fully active. Therefore, artificial membrane-like environments for protein folding are studied. Many approaches are developed to solve the membrane protein folding problems and based on manipulating the bilayer structures. Bacteriorhodopsin (bR) is the most widely studied membrane protein, consists of seven transmembrane helical segments and functions which can work as a proton pump in Halobacterium Salinarium. In the present study, the reversible control of bR conformation with simple light illumination provides a method to control membrane protein folding. The azobenzene-based photosurfactant undergoes a reversible photoisomerization upon illumination either visible (trans) or UV (cis) light. The trans isomer is relatively hydrophobic and planar than cis isomer. The phase behavior and bilayer structures are changed by the effect of photoresponsive surfactant. These strategies provide a convenient means to control membrane protein folding with light illumination. |
| Keyword | bacteriorhodopsin; photosurfactant; membrane protein; fold |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Contributing entity | University of Southern California |
| Rights | Chang, Chia Hao |
| Physical access | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@lib.usc.edu |
| Archival file | uscthesesreloadpub_Volume4/etd-ChangChiaH-932.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | CONTROLLING MEMBRANE PROTEIN FOLDING USING PHOTORESPONSIVE SURFACTANT by Chia Hao Chang A Dissertation Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree DOCTOR OF PHILOSOPHY (CHEMICAL ENGINEERING) August 2012 Copyright 2012 Chia Hao Chang |
