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LIST OF TABLES
Table 1: A. actinomycetemcomitans clinical isolates used in this work 6
Table 2: The growth parameters of A. actinomycetemcomitans D7S-1 and
D7SS of different dilutions 14
Table 3: Comparisons of doubling time and biofilm formation for the wildtype
fimbriated and smooth-colony nonfimbriated isogenic mutants of A.
actinomycetemcomitans 19
Table 4: Comparisons of growth parameters for A. actinomycetemcomitans
clinical isolates 22
Table 5: Comparisons of growth parameters for A. actinomycetemcomitans
clinical isolates grown in the chemically defined media by serotype 25
Table 6: Comparisons of growth parameters for A. actinomycetemcomitans
clinical isolates grown at 25oC 26
Table 7: Information of strain specific genes/islands examined in this study 33
Table 8: Comparison of doubling time for deleted/complemented
A. actinomycetemcomitans strain-specific genes in the standard
condition 39
Table 9: Comparison of biofilm formation for A. actinomycetemcomitans
strain-specific genes in the standard condition 40
Table 10: Effect of pH on growth of A. actinomycetemcomitans strains 43
Table 11: Comparison of the growth parameters of A. actinomycetemcomitans
strain-specific genes at pH=4.0 43
Table A. Composition of chemically defined media 49
Table B. Growth parameters of A. actinomycetemcomitans clinical isolates 51
Table C. Growth parameters of A. actinomycetemcomitans D7S-1 mutants 55
Table D. Growth parameters of A. actinomycetemcomitans reference strains 58
Object Description
| Title | Assessing the role of strain-specific DNA in the growth adn biofilm formation of aggregatibacter actinmycetemcomitans |
| Author | Jee, Sang Hyun "Jason" |
| Author email | sjee@usc.edu; shjee84@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Cranio-Facial Biology |
| School | School of Dentistry |
| Date defended/completed | 2011-03-30 |
| Date submitted | 2011 |
| Restricted until | Unrestricted |
| Date published | 2011-05-04 |
| Advisor (committee chair) | Chen, Casey |
| Advisor (committee member) | Paine, Michael,L. Sameshima, Glenn T. |
| Abstract | Strain-specific genes on genomic islands are often acquired by bacteria via horizontal gene transfer (HGT), which may enhance the fitness of the bacteria. Our laboratory has completed a project to compare the genome content of 14 A. actinomycetemcomitans strains recovered from periodontal health and disease. Numerous genomic islands of unknown functions were identified. Some may be nonfunctional vestiges of HGT-acquired genes that have undergone further mutations, while others may function to enhance the virulence of the strains. This study developed an approach to identify strain-specific genes that are involved in growth and biofilm formation of A. actinomycetemcomitans. Experimental variables that may affect the assay outcomes were evaluated. Different approaches for data analysis were also compared. Novel gene genetic protocols were used to construct mutants for A. actinomycetemcomitans strain D7S-1. These mutants were tested for their doubling time and biofilm formation. Mutants with a deletion of a ~5 Kb genomic island (designated as “285-island”) exhibited faster doubling time than the wildtype D7S-1 or other mutants. Deletion mutants restored with a copy of the 285-island exhibited doubling time similar to that of wildtype strain D7S-1. Growth of strain-specific genes in pH 4.0 was also tested and two groups of the mutants were distinguished based on their ability to grow at pH 4.0. This is the first study that demonstrated a function of any strain-specific genomic island (except serotype-specific gene cluster) of A. actinomycetemcomitans, and validated our approach to screen for functionally important genomic islands among A. actinomycetemcomitans strains. |
| Keyword | peridontal disease; dentistry; growth; biofilmActinomycetemcomitans; doubling time; acidic condition |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m3900 |
| Contributing entity | University of Southern California |
| Rights | Jee, Sang Hyun "Jason" |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@lib.usc.edu |
| Filename | etd-Jee-4154 |
| Archival file | uscthesesreloadpub_Volume32/etd-Jee-4154.pdf |
Description
| Title | Page 5 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | v LIST OF TABLES Table 1: A. actinomycetemcomitans clinical isolates used in this work 6 Table 2: The growth parameters of A. actinomycetemcomitans D7S-1 and D7SS of different dilutions 14 Table 3: Comparisons of doubling time and biofilm formation for the wildtype fimbriated and smooth-colony nonfimbriated isogenic mutants of A. actinomycetemcomitans 19 Table 4: Comparisons of growth parameters for A. actinomycetemcomitans clinical isolates 22 Table 5: Comparisons of growth parameters for A. actinomycetemcomitans clinical isolates grown in the chemically defined media by serotype 25 Table 6: Comparisons of growth parameters for A. actinomycetemcomitans clinical isolates grown at 25oC 26 Table 7: Information of strain specific genes/islands examined in this study 33 Table 8: Comparison of doubling time for deleted/complemented A. actinomycetemcomitans strain-specific genes in the standard condition 39 Table 9: Comparison of biofilm formation for A. actinomycetemcomitans strain-specific genes in the standard condition 40 Table 10: Effect of pH on growth of A. actinomycetemcomitans strains 43 Table 11: Comparison of the growth parameters of A. actinomycetemcomitans strain-specific genes at pH=4.0 43 Table A. Composition of chemically defined media 49 Table B. Growth parameters of A. actinomycetemcomitans clinical isolates 51 Table C. Growth parameters of A. actinomycetemcomitans D7S-1 mutants 55 Table D. Growth parameters of A. actinomycetemcomitans reference strains 58 |
