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78
dynamics, as demonstrated previously by analysis of replication initiation structures with 2D-gels (O'Neill et al, 2007). Analysis of budding kinetics also supports the conclusion that pph3Δ cells progress through the G1-S transition like wild-type cells (Fig. 19B). Thus, replication initiation at early origins appears normal in the absence of PPH3.
The progression of replication forks away from ARS607 is impaired in the absence of PPH3. During the 45-60 minute period, little BrdU incorporation is observed at ARS607. However, two flanking peaks of BrdU incorporation are observed, reflecting DNA synthesis at each replication fork emanating from ARS607 (Fig. 19A, the leftward-moving fork partially converges with the rightward-moving fork from ARS606). These patterns appear similar in wild-type and pph3Δ cells; however, the rightward-moving ARS607 replication fork appears to progress ~10kb further from the origin in wild-type cells. The more distant progression of this replication fork in wild-type cells becomes more pronounced over the time-course. By the 105-120 minute interval, BrdU incorporation occurs over a large region of chromosome VI extending ~65kb from ARS607 toward the telomere in wild-type cells, whereas BrdU incorporation in pph3Δ cells extends only ~35kb from ARS607. Wild-type cells also complete replication of the region between ARS606 and ARS607 by ~90 minutes, while BrdU incorporation in this region continues through 120 minutes in the pph3Δ cells.
The chromosome VI results are supported by analysis of the expanded chromosome III replicon, demonstrating limited progression of the leftward-moving replication fork from ARS306 in pph3Δ cells compared with wild-type cells (Fig. 20).
Object Description
| Title | The intra-S phase checkpoint and its effect on replication fork dynamics in saccharomyces cerevisiae |
| Author | Szyjka, Shawn Joseph |
| Author email | sszyjka@gmail.com; hammonds77@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Molecular Biology |
| School | College of Letters, Arts and Sciences |
| Date defended/completed | 2008-07-25 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-10-21 |
| Advisor (committee chair) | Aparicio, Oscar |
| Advisor (committee member) |
Forsburg, Susan Finkel, Steven E. Qin, Peter Z. Rice, Judd C. |
| Abstract | Duplication of a cell's genetic material is of paramount importance. This task must be completed accurately, efficiently and occur once and only once within any given cell cycle. Origin "firing", which occurs according to a temporal program, results in the establishment of bidirectional replication forks. As replication forks traverse the chromosomal landscape, their stability is threatened by endogenous "obstacles" such as heavily transcribed tRNAs, protein-DNA complexes and "replication slow zones". In addition, fork stability can also be threatened by genotoxic agents. During S phase, in response to genotoxin-induced stress, the cell activates the intra-S phase checkpoint. The intra-S phase checkpoint is comprised of three major groups of proteins: sensors, adaptors and effectors. Sensor proteins detect problems at the replication fork and elicit a phosphorylation cascade that is mediated by adaptor proteins and results in the activation of effector kinases. Together, these proteins act to inhibit late origin firing, slow cell cycle progression, upregulate DNA repair genes, and stabilize replication forks until the stress has been alleviated.; In response to nucleotide depletion, the adaptor protein, Mrc1 (Mediator of the Replication Checkpoint), mediates a checkpoint signal that activates the effector kinase, Rad53. In addition to its checkpoint role, Mrc1 also appears to play a role in unperturbed DNA synthesis. Mrc1 travels with replication forks and mrc1delta cells display slowed bulk DNA synthesis. This phenotype could be due to decreased origin firing, increased fork pausing at endogenous "obstacles", or overall defective fork progression. Using two-dimensional (2D) gel electrophoresis of replication intermediates, we demonstrate that mrc1delta cells are not defective in fork pausing at tRNAs and that Mrc1's replication function is required for efficient progression of replication forks throughout the genome. We hypothesize that Mrc1 maintains association between polymerases and helicases at the replication fork, which results in efficient fork progression.; In response to MMS-induced DNA damage, Rad53 plays a vital role in replication fork stabilization. It is thought that Rad53 maintains the association of replisome components so that forks are poised for efficient restart upon checkpoint deactivation. Despite a wealth of knowledge with respect to checkpoint activation, relatively little is known about checkpoint deactivation and how replication forks restart. Previous work has suggested that replication fork progression along an MMS-damaged template is independent of the checkpoint. However, recent studies lacking Psy2-Pph3, a Rad53 phosphatase responsible for Rad53 deactivation after DNA damage, suggest otherwise, as the completion of S-phase after DNA damage is delayed in the absence of Psy2-Pph3. We have examined the role of Rad53 in the control of replication fork restart by monitoring BrdU incorporation at replication forks in the presence of DNA damage and during recovery from damage. We find that Rad53 deactivation is a key requirement for replication fork restart as cells lacking PPH3 are defective in fork progression in the presence of DNA damage and are delayed in replication restart during recovery. In addition, dominant-negative inactivation of Rad53 in pph3delta cells, enables replication fork restart, arguing that deactivation of Rad53 is sufficient for replication fork restart. Deletion of PTC2, which encodes a second, unrelated Rad53 phosphatase, in addition to PPH3, completely eliminates replication fork progression under DNA damaging conditions and results in lethality. These findings suggest that replication fork stabilization and restart involve a cycle of Rad53 activation and deactivation, and that at least two distinct phosphatases are responsible for regulating this process. |
| Keyword | DNA replication; DNA damage; replication fork; DNA repair; cell cycle checkpoint; phosphatase; Rad53; Mrc1; BrdU; microarray; Claspin; replication fork progression; Rrm3; Pph3; 2-D gel electro |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m1688 |
| Contributing entity | University of Southern California |
| Rights | Szyjka, Shawn Joseph |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@lib.usc.edu |
| Filename | etd-Szyjka-2341 |
| Archival file | uscthesesreloadpub_Volume32/etd-Szyjka-2341.pdf |
Description
| Title | Page 93 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | 78 dynamics, as demonstrated previously by analysis of replication initiation structures with 2D-gels (O'Neill et al, 2007). Analysis of budding kinetics also supports the conclusion that pph3Δ cells progress through the G1-S transition like wild-type cells (Fig. 19B). Thus, replication initiation at early origins appears normal in the absence of PPH3. The progression of replication forks away from ARS607 is impaired in the absence of PPH3. During the 45-60 minute period, little BrdU incorporation is observed at ARS607. However, two flanking peaks of BrdU incorporation are observed, reflecting DNA synthesis at each replication fork emanating from ARS607 (Fig. 19A, the leftward-moving fork partially converges with the rightward-moving fork from ARS606). These patterns appear similar in wild-type and pph3Δ cells; however, the rightward-moving ARS607 replication fork appears to progress ~10kb further from the origin in wild-type cells. The more distant progression of this replication fork in wild-type cells becomes more pronounced over the time-course. By the 105-120 minute interval, BrdU incorporation occurs over a large region of chromosome VI extending ~65kb from ARS607 toward the telomere in wild-type cells, whereas BrdU incorporation in pph3Δ cells extends only ~35kb from ARS607. Wild-type cells also complete replication of the region between ARS606 and ARS607 by ~90 minutes, while BrdU incorporation in this region continues through 120 minutes in the pph3Δ cells. The chromosome VI results are supported by analysis of the expanded chromosome III replicon, demonstrating limited progression of the leftward-moving replication fork from ARS306 in pph3Δ cells compared with wild-type cells (Fig. 20). |
