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Ramadan, N., I. Flockhart, et al. (2007). "Design and implementation of high-throughput
RNAi screens in cultured Drosophila cells." Nat Protoc 2(9): 2245-64.
Reiter, L. T., L. Potocki, et al. (2001). "A systematic analysis of human disease-associated
gene sequences in Drosophila melanogaster." Genome Res 11(6): 1114-25.
Ren, C., P. Webster, et al. (2007). "Increased internal and external bacterial load during
Drosophila aging without life-span trade-off." Cell Metab 6(2): 144-52.
Ring, B. C., H. W. Bass, et al. (2000). "Construction and transposition of a 100-kilobase
extended P element in Drosophila." Genome Res 10(10): 1605-16.
Rio, D. C. (1990). "Molecular mechanisms regulating Drosophila P element
transposition." Annu Rev Genet 24: 543-78.
Rio, D. C. and G. M. Rubin (1988). "Identification and purification of a Drosophila
protein that binds to the terminal 31-base-pair inverted repeats of the P transposable
element." Proc Natl Acad Sci U S A 85(23): 8929-33.
Robertson, H. M., C. R. Preston, et al. (1988). "A stable genomic source of P element
transposase in Drosophila melanogaster." Genetics 118(3): 461-70.
Rocnik, E. F., P. Liu, et al. (2006). "The novel SPARC family member SMOC-2
potentiates angiogenic growth factor activity." J Biol Chem 281(32): 22855-64.
Rogers, S. L. and G. C. Rogers (2008). "Culture of Drosophila S2 cells and their use for
RNAi-mediated loss-of-function studies and immunofluorescence microscopy." Nat
Protoc 3(4): 606-11.
Roman, G., K. Endo, et al. (2001). "P[Switch], a system for spatial and temporal control
of gene expression in Drosophila melanogaster." Proc Natl Acad Sci U S A 98(22):
12602-7.
Rubin, G. M. and A. C. Spradling (1982). "Genetic transformation of Drosophila with
transposable element vectors." Science 218: 348-353.
Rubin, G. M. and A. C. Spradling (1982). "Genetic transformation of Drosophila with
transposable element vectors." Science 218(4570): 348-53.
Rubin, G. M. and A. C. Spradling (1983). "Vectors for P element-mediated gene transfer
in Drosophila." Nucleic Acids Res 11(18): 6341-51.
Sellge, G., A. Lorentz, et al. (2004). "Human intestinal fibroblasts prevent apoptosis in
human intestinal mast cells by a mechanism independent of stem cell factor, IL-3, IL-4,
and nerve growth factor." J Immunol 172(1): 260-7.
Object Description
| Title | Characterization of Drosophila longevity and fecundity regulating genes |
| Author | Li, Yishi |
| Author email | yishili@usc.edu; yishili@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Molecular & Computational Biology |
| School | College of Letters, Arts and Sciences |
| Date defended/completed | 2008-08-19 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-10-31 |
| Advisor (committee chair) | Tower, John |
| Advisor (committee member) |
Finkel, Steven E. Aparicio, Oscar Martin Longo, Valter D Comai, Lucio |
| Abstract | The regulation of Drosophila melanogaster longevity and fecundity involves many factors. Longevity is governed by oxidative stress, stem cell loss, dietary restriction, the insulin/IGF-1 pathway, and other factors. Fecundity is also regulated by multiple tissues and factors, including the germline stem cells and stem cell niche, the fat body, yolk proteins, and sex peptides. The fecundity of wild type female Drosophila gradually declines during aging, suggesting a common pathway regulating longevity and fecundity machinery. Since both mechanisms involve multiple factors, sorting through the Gordian’s knot is a formidable task. Using a PdL mutagenesis approach, I screened for a specific phenotype in thousands of independent mutant strains to examine both regulatory networks simultaneously. Two novel genes, magu and hebe, were identified and characterized to regulate longevity and fecundity. While Drosophila lifespan was extended upon the induction of these genes, fecundity increase requires that the gene induction be in an ideal range to show the expected phenotypic change. I also performed several other projects, including studying the lifespan extension effect of dIAP2, characterization of a Drosophila gut driver strain, and intra-abdominal RNAi injection in adult Drosophila. These projects provided us insight on longevity, fecundity, anti-apoptosis, stem cell biology, RNAi and other aspects of Drosophila research. In sum, Drosophila melanogaster, as a model organism for molecular biology and genetics study, will continue to contribute to the scientific community. |
| Keyword | Drosophila; longevity; fecundity |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m1735 |
| Contributing entity | University of Southern California |
| Rights | Li, Yishi |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@lib.usc.edu |
| Filename | etd-Li-2382 |
| Archival file | uscthesesreloadpub_Volume44/etd-Li-2382.pdf |
Description
| Title | Page 146 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | 136 Ramadan, N., I. Flockhart, et al. (2007). "Design and implementation of high-throughput RNAi screens in cultured Drosophila cells." Nat Protoc 2(9): 2245-64. Reiter, L. T., L. Potocki, et al. (2001). "A systematic analysis of human disease-associated gene sequences in Drosophila melanogaster." Genome Res 11(6): 1114-25. Ren, C., P. Webster, et al. (2007). "Increased internal and external bacterial load during Drosophila aging without life-span trade-off." Cell Metab 6(2): 144-52. Ring, B. C., H. W. Bass, et al. (2000). "Construction and transposition of a 100-kilobase extended P element in Drosophila." Genome Res 10(10): 1605-16. Rio, D. C. (1990). "Molecular mechanisms regulating Drosophila P element transposition." Annu Rev Genet 24: 543-78. Rio, D. C. and G. M. Rubin (1988). "Identification and purification of a Drosophila protein that binds to the terminal 31-base-pair inverted repeats of the P transposable element." Proc Natl Acad Sci U S A 85(23): 8929-33. Robertson, H. M., C. R. Preston, et al. (1988). "A stable genomic source of P element transposase in Drosophila melanogaster." Genetics 118(3): 461-70. Rocnik, E. F., P. Liu, et al. (2006). "The novel SPARC family member SMOC-2 potentiates angiogenic growth factor activity." J Biol Chem 281(32): 22855-64. Rogers, S. L. and G. C. Rogers (2008). "Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy." Nat Protoc 3(4): 606-11. Roman, G., K. Endo, et al. (2001). "P[Switch], a system for spatial and temporal control of gene expression in Drosophila melanogaster." Proc Natl Acad Sci U S A 98(22): 12602-7. Rubin, G. M. and A. C. Spradling (1982). "Genetic transformation of Drosophila with transposable element vectors." Science 218: 348-353. Rubin, G. M. and A. C. Spradling (1982). "Genetic transformation of Drosophila with transposable element vectors." Science 218(4570): 348-53. Rubin, G. M. and A. C. Spradling (1983). "Vectors for P element-mediated gene transfer in Drosophila." Nucleic Acids Res 11(18): 6341-51. Sellge, G., A. Lorentz, et al. (2004). "Human intestinal fibroblasts prevent apoptosis in human intestinal mast cells by a mechanism independent of stem cell factor, IL-3, IL-4, and nerve growth factor." J Immunol 172(1): 260-7. |
