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128
Dzitoyeva, S., N. Dimitrijevic, et al. (2001). "Intra-abdominal injection of double-stranded
RNA into anesthetized adult Drosophila triggers RNA interference in the
central nervous system." Mol Psychiatry 6(6): 665-70.
Dzitoyeva, S., N. Dimitrijevic, et al. (2003). "Identification of a novel Drosophila gene,
beltless, using injectable embryonic and adult RNA interference (RNAi)." BMC
Genomics 4(1): 33.
Edwards, A. C., S. M. Rollmann, et al. (2006). "Quantitative genomics of aggressive
behavior in Drosophila melanogaster." PLoS Genet 2(9): e154.
Egger, B., J. Q. Boone, et al. (2007). "Regulation of spindle orientation and neural stem
cell fate in the Drosophila optic lobe." Neural Develop 2: 1.
Finn, R. D., J. Mistry, et al. (2006). "Pfam: clans, web tools and services." Nucleic Acids
Res 34(Database issue): D247-51.
Fire, A. (1999). "RNA-triggered gene silencing." Trends Genet 15(9): 358-63.
Fire, A., S. Xu, et al. (1998). "Potent and specific genetic interference by double-stranded
RNA in Caenorhabditis elegans." Nature 391(6669): 806-11.
Flockhart, I., M. Booker, et al. (2006). "FlyRNAi: the Drosophila RNAi screening center
database." Nucleic Acids Res 34(Database issue): D489-94.
Ford, D., N. Hoe, et al. (2007). "Alteration of Drosophila lifespan using conditional,
tissue-specific expression of transgenes triggered by doxycyline or
RU486/Mifepristone." Exp Gerontol 42(6): 483-97.
Fraser, A. G., R. S. Kamath, et al. (2000). "Functional genomic analysis of C. elegans
chromosome I by systematic RNA interference." Nature 408(6810): 325-30.
Gems, D., A. J. Sutton, et al. (1998). "Two pleiotropic classes of daf-2 mutation affect
larval arrest, adult behavior, reproduction and longevity in Caenorhabditis elegans."
Genetics 150(1): 129-55.
Georgel, P., S. Naitza, et al. (2001). "Drosophila immune deficiency (IMD) is a death
domain protein that activates antibacterial defense and can promote apoptosis." Dev Cell
1(4): 503-14.
Gesellchen, V., D. Kuttenkeuler, et al. (2005). "An RNA interference screen identifies
Inhibitor of Apoptosis Protein 2 as a regulator of innate immune signalling in
Drosophila." EMBO Rep 6(10): 979-84.
Object Description
| Title | Characterization of Drosophila longevity and fecundity regulating genes |
| Author | Li, Yishi |
| Author email | yishili@usc.edu; yishili@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Molecular & Computational Biology |
| School | College of Letters, Arts and Sciences |
| Date defended/completed | 2008-08-19 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-10-31 |
| Advisor (committee chair) | Tower, John |
| Advisor (committee member) |
Finkel, Steven E. Aparicio, Oscar Martin Longo, Valter D Comai, Lucio |
| Abstract | The regulation of Drosophila melanogaster longevity and fecundity involves many factors. Longevity is governed by oxidative stress, stem cell loss, dietary restriction, the insulin/IGF-1 pathway, and other factors. Fecundity is also regulated by multiple tissues and factors, including the germline stem cells and stem cell niche, the fat body, yolk proteins, and sex peptides. The fecundity of wild type female Drosophila gradually declines during aging, suggesting a common pathway regulating longevity and fecundity machinery. Since both mechanisms involve multiple factors, sorting through the Gordian’s knot is a formidable task. Using a PdL mutagenesis approach, I screened for a specific phenotype in thousands of independent mutant strains to examine both regulatory networks simultaneously. Two novel genes, magu and hebe, were identified and characterized to regulate longevity and fecundity. While Drosophila lifespan was extended upon the induction of these genes, fecundity increase requires that the gene induction be in an ideal range to show the expected phenotypic change. I also performed several other projects, including studying the lifespan extension effect of dIAP2, characterization of a Drosophila gut driver strain, and intra-abdominal RNAi injection in adult Drosophila. These projects provided us insight on longevity, fecundity, anti-apoptosis, stem cell biology, RNAi and other aspects of Drosophila research. In sum, Drosophila melanogaster, as a model organism for molecular biology and genetics study, will continue to contribute to the scientific community. |
| Keyword | Drosophila; longevity; fecundity |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m1735 |
| Contributing entity | University of Southern California |
| Rights | Li, Yishi |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@lib.usc.edu |
| Filename | etd-Li-2382 |
| Archival file | uscthesesreloadpub_Volume44/etd-Li-2382.pdf |
Description
| Title | Page 138 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | 128 Dzitoyeva, S., N. Dimitrijevic, et al. (2001). "Intra-abdominal injection of double-stranded RNA into anesthetized adult Drosophila triggers RNA interference in the central nervous system." Mol Psychiatry 6(6): 665-70. Dzitoyeva, S., N. Dimitrijevic, et al. (2003). "Identification of a novel Drosophila gene, beltless, using injectable embryonic and adult RNA interference (RNAi)." BMC Genomics 4(1): 33. Edwards, A. C., S. M. Rollmann, et al. (2006). "Quantitative genomics of aggressive behavior in Drosophila melanogaster." PLoS Genet 2(9): e154. Egger, B., J. Q. Boone, et al. (2007). "Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe." Neural Develop 2: 1. Finn, R. D., J. Mistry, et al. (2006). "Pfam: clans, web tools and services." Nucleic Acids Res 34(Database issue): D247-51. Fire, A. (1999). "RNA-triggered gene silencing." Trends Genet 15(9): 358-63. Fire, A., S. Xu, et al. (1998). "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans." Nature 391(6669): 806-11. Flockhart, I., M. Booker, et al. (2006). "FlyRNAi: the Drosophila RNAi screening center database." Nucleic Acids Res 34(Database issue): D489-94. Ford, D., N. Hoe, et al. (2007). "Alteration of Drosophila lifespan using conditional, tissue-specific expression of transgenes triggered by doxycyline or RU486/Mifepristone." Exp Gerontol 42(6): 483-97. Fraser, A. G., R. S. Kamath, et al. (2000). "Functional genomic analysis of C. elegans chromosome I by systematic RNA interference." Nature 408(6810): 325-30. Gems, D., A. J. Sutton, et al. (1998). "Two pleiotropic classes of daf-2 mutation affect larval arrest, adult behavior, reproduction and longevity in Caenorhabditis elegans." Genetics 150(1): 129-55. Georgel, P., S. Naitza, et al. (2001). "Drosophila immune deficiency (IMD) is a death domain protein that activates antibacterial defense and can promote apoptosis." Dev Cell 1(4): 503-14. Gesellchen, V., D. Kuttenkeuler, et al. (2005). "An RNA interference screen identifies Inhibitor of Apoptosis Protein 2 as a regulator of innate immune signalling in Drosophila." EMBO Rep 6(10): 979-84. |
