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60
Figure 13: The lifespan assay of Ban PdL mutant performed on two different food
recipes. The lifespan assay was performed as described above. The lifespan curves are
labeled as above. Different food recipes with different +/-DOX group are labeled by
different colors and dots. For food recipe comparison, see table 10. New food recipe
with +DOX is labeled red. New food recipe with –DOX is labeled blue. Old food recipe
with +DOX is labeled purple. Old food recipe with –DOX is labeled green. Only female
progeny were assayed for lifespan in this round. A: Or-R lifespan curve; B: Ban PdL
mutant lifespan curve
Figure 14: The repeat round of the lifespan assay of Ban PdL mutant. The lifespan assay
was performed as described above. The lifespan curves are labeled as above. A: W1118
male; B: P{PdL}ban PdL mutant male; C: W1118 female; D: P{PdL}ban PdL mutant
female
Object Description
| Title | Characterization of Drosophila longevity and fecundity regulating genes |
| Author | Li, Yishi |
| Author email | yishili@usc.edu; yishili@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Molecular & Computational Biology |
| School | College of Letters, Arts and Sciences |
| Date defended/completed | 2008-08-19 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-10-31 |
| Advisor (committee chair) | Tower, John |
| Advisor (committee member) |
Finkel, Steven E. Aparicio, Oscar Martin Longo, Valter D Comai, Lucio |
| Abstract | The regulation of Drosophila melanogaster longevity and fecundity involves many factors. Longevity is governed by oxidative stress, stem cell loss, dietary restriction, the insulin/IGF-1 pathway, and other factors. Fecundity is also regulated by multiple tissues and factors, including the germline stem cells and stem cell niche, the fat body, yolk proteins, and sex peptides. The fecundity of wild type female Drosophila gradually declines during aging, suggesting a common pathway regulating longevity and fecundity machinery. Since both mechanisms involve multiple factors, sorting through the Gordian’s knot is a formidable task. Using a PdL mutagenesis approach, I screened for a specific phenotype in thousands of independent mutant strains to examine both regulatory networks simultaneously. Two novel genes, magu and hebe, were identified and characterized to regulate longevity and fecundity. While Drosophila lifespan was extended upon the induction of these genes, fecundity increase requires that the gene induction be in an ideal range to show the expected phenotypic change. I also performed several other projects, including studying the lifespan extension effect of dIAP2, characterization of a Drosophila gut driver strain, and intra-abdominal RNAi injection in adult Drosophila. These projects provided us insight on longevity, fecundity, anti-apoptosis, stem cell biology, RNAi and other aspects of Drosophila research. In sum, Drosophila melanogaster, as a model organism for molecular biology and genetics study, will continue to contribute to the scientific community. |
| Keyword | Drosophila; longevity; fecundity |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m1735 |
| Contributing entity | University of Southern California |
| Rights | Li, Yishi |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@lib.usc.edu |
| Filename | etd-Li-2382 |
| Archival file | uscthesesreloadpub_Volume44/etd-Li-2382.pdf |
Description
| Title | Page 70 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | 60 Figure 13: The lifespan assay of Ban PdL mutant performed on two different food recipes. The lifespan assay was performed as described above. The lifespan curves are labeled as above. Different food recipes with different +/-DOX group are labeled by different colors and dots. For food recipe comparison, see table 10. New food recipe with +DOX is labeled red. New food recipe with –DOX is labeled blue. Old food recipe with +DOX is labeled purple. Old food recipe with –DOX is labeled green. Only female progeny were assayed for lifespan in this round. A: Or-R lifespan curve; B: Ban PdL mutant lifespan curve Figure 14: The repeat round of the lifespan assay of Ban PdL mutant. The lifespan assay was performed as described above. The lifespan curves are labeled as above. A: W1118 male; B: P{PdL}ban PdL mutant male; C: W1118 female; D: P{PdL}ban PdL mutant female |
