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59
therefore it did not show the over-expression of gene bantam upon DOX induction (Data
not shown).
Figure 11: The PdL insertion sequence map for screen 2 Ban PdL mutant strain. PdL
insertion is labeled by the inverted triangle. The length of the chromosome is labeled by
5kb major tick marks and 1kb minor tick marks. The genes on either strand of the
chromosome are labeled by wide gray arrows pointing 5’ to 3’.
Figure 12: The parallel fecundity and lifespan test result curves for screen 2 Ban PdL
mutant strain. The fecundity and lifespan assays were performed as previously
described. The fecundity and lifespan curves are labeled as above. Only female progeny
were assayed for lifespan in this round. A: Or-R fecundity curve; B: Or-R lifespan
curve; C: Ban PdL mutant fecundity curve; D: Ban PdL mutant lifespan curve
Object Description
| Title | Characterization of Drosophila longevity and fecundity regulating genes |
| Author | Li, Yishi |
| Author email | yishili@usc.edu; yishili@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Molecular & Computational Biology |
| School | College of Letters, Arts and Sciences |
| Date defended/completed | 2008-08-19 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-10-31 |
| Advisor (committee chair) | Tower, John |
| Advisor (committee member) |
Finkel, Steven E. Aparicio, Oscar Martin Longo, Valter D Comai, Lucio |
| Abstract | The regulation of Drosophila melanogaster longevity and fecundity involves many factors. Longevity is governed by oxidative stress, stem cell loss, dietary restriction, the insulin/IGF-1 pathway, and other factors. Fecundity is also regulated by multiple tissues and factors, including the germline stem cells and stem cell niche, the fat body, yolk proteins, and sex peptides. The fecundity of wild type female Drosophila gradually declines during aging, suggesting a common pathway regulating longevity and fecundity machinery. Since both mechanisms involve multiple factors, sorting through the Gordian’s knot is a formidable task. Using a PdL mutagenesis approach, I screened for a specific phenotype in thousands of independent mutant strains to examine both regulatory networks simultaneously. Two novel genes, magu and hebe, were identified and characterized to regulate longevity and fecundity. While Drosophila lifespan was extended upon the induction of these genes, fecundity increase requires that the gene induction be in an ideal range to show the expected phenotypic change. I also performed several other projects, including studying the lifespan extension effect of dIAP2, characterization of a Drosophila gut driver strain, and intra-abdominal RNAi injection in adult Drosophila. These projects provided us insight on longevity, fecundity, anti-apoptosis, stem cell biology, RNAi and other aspects of Drosophila research. In sum, Drosophila melanogaster, as a model organism for molecular biology and genetics study, will continue to contribute to the scientific community. |
| Keyword | Drosophila; longevity; fecundity |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m1735 |
| Contributing entity | University of Southern California |
| Rights | Li, Yishi |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@lib.usc.edu |
| Filename | etd-Li-2382 |
| Archival file | uscthesesreloadpub_Volume44/etd-Li-2382.pdf |
Description
| Title | Page 69 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | 59 therefore it did not show the over-expression of gene bantam upon DOX induction (Data not shown). Figure 11: The PdL insertion sequence map for screen 2 Ban PdL mutant strain. PdL insertion is labeled by the inverted triangle. The length of the chromosome is labeled by 5kb major tick marks and 1kb minor tick marks. The genes on either strand of the chromosome are labeled by wide gray arrows pointing 5’ to 3’. Figure 12: The parallel fecundity and lifespan test result curves for screen 2 Ban PdL mutant strain. The fecundity and lifespan assays were performed as previously described. The fecundity and lifespan curves are labeled as above. Only female progeny were assayed for lifespan in this round. A: Or-R fecundity curve; B: Or-R lifespan curve; C: Ban PdL mutant fecundity curve; D: Ban PdL mutant lifespan curve |
