Page 34 |
Save page Remove page | Previous | 34 of 150 | Next |
|
small (250x250 max)
medium (500x500 max)
Large (1000x1000 max)
Extra Large
large ( > 500x500)
Full Resolution
All (PDF)
|
This page
All
|
24
the experiment was performed until all the females were dead. The number of pupae in
each vial was counted 10 days after each transfer. For each vial, the number of pupae
was divided by the number of females in that specific period to yield progeny per
female. The average pupae number per female +/-SEM was calculated across the 10
replicate vials. A preliminary version of these data was presented in a review article
(Waskar, Li et al. 2005), and the data are included here with further statistical analysis
for comparison purposes.
Genetic screen for new PdL mutations
Virgin flies from strain PdL45C1 were crossed to males from strain delta2-3. The male
progeny containing both the PdL45C1 insertion and delta2-3 were selected by their
phenotypic markers and crossed to virgins of strain rtTA(3)E2. The virgin progeny flies
containing a new PdL insertion and rtTA(3)E2 have the ability to over-express the gene
immediately downstream of the PdL insertion, and ~8,000 of these mutants were
generated and analyzed. (See figure 3 for cross design detail.) Each individual mutant
female was combined with 3 young Oregon-R male flies and cultured on -DOX food for
the first 35 days of their lifespan. During this process the flies were transferred to fresh
food vials every two days. At the end of the 35 day period, the flies were then cultured
on –DOX food for 4 days (“Time Period 1”). The flies were then cultured on +DOX
food for 6 days to allow any gene over-expression and phenotypes to become apparent.
The flies were then cultured on +DOX food for an additional 4 days (“Time Period 2”).
Object Description
| Title | Characterization of Drosophila longevity and fecundity regulating genes |
| Author | Li, Yishi |
| Author email | yishili@usc.edu; yishili@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Molecular & Computational Biology |
| School | College of Letters, Arts and Sciences |
| Date defended/completed | 2008-08-19 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-10-31 |
| Advisor (committee chair) | Tower, John |
| Advisor (committee member) |
Finkel, Steven E. Aparicio, Oscar Martin Longo, Valter D Comai, Lucio |
| Abstract | The regulation of Drosophila melanogaster longevity and fecundity involves many factors. Longevity is governed by oxidative stress, stem cell loss, dietary restriction, the insulin/IGF-1 pathway, and other factors. Fecundity is also regulated by multiple tissues and factors, including the germline stem cells and stem cell niche, the fat body, yolk proteins, and sex peptides. The fecundity of wild type female Drosophila gradually declines during aging, suggesting a common pathway regulating longevity and fecundity machinery. Since both mechanisms involve multiple factors, sorting through the Gordian’s knot is a formidable task. Using a PdL mutagenesis approach, I screened for a specific phenotype in thousands of independent mutant strains to examine both regulatory networks simultaneously. Two novel genes, magu and hebe, were identified and characterized to regulate longevity and fecundity. While Drosophila lifespan was extended upon the induction of these genes, fecundity increase requires that the gene induction be in an ideal range to show the expected phenotypic change. I also performed several other projects, including studying the lifespan extension effect of dIAP2, characterization of a Drosophila gut driver strain, and intra-abdominal RNAi injection in adult Drosophila. These projects provided us insight on longevity, fecundity, anti-apoptosis, stem cell biology, RNAi and other aspects of Drosophila research. In sum, Drosophila melanogaster, as a model organism for molecular biology and genetics study, will continue to contribute to the scientific community. |
| Keyword | Drosophila; longevity; fecundity |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m1735 |
| Contributing entity | University of Southern California |
| Rights | Li, Yishi |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@lib.usc.edu |
| Filename | etd-Li-2382 |
| Archival file | uscthesesreloadpub_Volume44/etd-Li-2382.pdf |
Description
| Title | Page 34 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | 24 the experiment was performed until all the females were dead. The number of pupae in each vial was counted 10 days after each transfer. For each vial, the number of pupae was divided by the number of females in that specific period to yield progeny per female. The average pupae number per female +/-SEM was calculated across the 10 replicate vials. A preliminary version of these data was presented in a review article (Waskar, Li et al. 2005), and the data are included here with further statistical analysis for comparison purposes. Genetic screen for new PdL mutations Virgin flies from strain PdL45C1 were crossed to males from strain delta2-3. The male progeny containing both the PdL45C1 insertion and delta2-3 were selected by their phenotypic markers and crossed to virgins of strain rtTA(3)E2. The virgin progeny flies containing a new PdL insertion and rtTA(3)E2 have the ability to over-express the gene immediately downstream of the PdL insertion, and ~8,000 of these mutants were generated and analyzed. (See figure 3 for cross design detail.) Each individual mutant female was combined with 3 young Oregon-R male flies and cultured on -DOX food for the first 35 days of their lifespan. During this process the flies were transferred to fresh food vials every two days. At the end of the 35 day period, the flies were then cultured on –DOX food for 4 days (“Time Period 1”). The flies were then cultured on +DOX food for 6 days to allow any gene over-expression and phenotypes to become apparent. The flies were then cultured on +DOX food for an additional 4 days (“Time Period 2”). |
