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HUMAN HISTOLOGICAL ANALYSIS OF AUTOGENOUS BLOCK GRAFTS
by
Andrew Kim
____________________________________________________
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(CRANIOFACIAL BIOLOGY)
December 2012
Copyright 2012 Andrew Kim
Object Description
| Title | Human histological analysis of autogenous block grafts |
| Author | Kim, Andrew |
| Author email | kimandre@usc.edu;andykdds@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Cranio-Facial Biology |
| School | School of Dentistry |
| Date defended/completed | 2012-07-17 |
| Date submitted | 2012-08-31 |
| Date approved | 2012-09-01 |
| Restricted until | 2012-09-01 |
| Date published | 2012-09-01 |
| Advisor (committee chair) | Zadeh, Homayoun H. |
| Advisor (committee member) |
Rich, Sandra Navazesh, Mahvash |
| Abstract | Background: Autogenous block bone grafting is a treatment option for ridge deformities. Currently, the healing process of autogenous bone grafting in humans has not been histologically reported and the vitality of bone after graft placement has been challenged. ❧ Objective: The purpose of this study is to histologically evaluate vitality of autogenous bone grafts in patients who have been treated for ridge deformities in preparation for implant placement. ❧ Materials and Methods: At 4, 5, and 7 months after bone augmentation and at the time of implant placement, a core of 2x3 mm bone graft was trephined for histological analysis (n=4; mean age 37.3 years; 1:1 male/female). Samples were fixated with 10% neutral buffered formalin (NBF), decalcified, embedded in paraffin, and stained with Hemotoxylin & Eosin. Angiogenesis, bone vitality (presence of osteocytes within lacunae), and the Haversian systems were examined. ❧ Results: Bleeding was observed at the time of sample collection. All surgeries healed uneventfully and all implants were stabilized at 50 Ncm. Diffuse angiogenesis and cellular vitality were identified in all sections. Osteogenesis resulted in vital bone formation, which was demonstrated by the presence of osteocytes within lacunae, lining osteoblasts, different staining color, immature organization, and demarcation lines. Vital bone was adjacent to blood supply and was amalgated with graft bone. Symphysis grafts exhibited more angiogenesis and osteogenesis than ramus grafts. ❧ Conclusion: This human histologic study demonstrated that autogenous bone grafting leads to vital bone, confirmed by bleeding at the time of sample collection, histologic detection of angiogenesis, osteogenesis, and diffuse cellular activity. Remodeling was ongoing at 4, 5, 7 months and was more pronounced in symphysis grafts than in ramus grafts. Further studies are needed to investigate the mechanism of graft healing in humans and factors that influence bone graft remodeling. |
| Keyword | autogenous bone; histology; ramus; symphysis |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Contributing entity | University of Southern California |
| Rights | Kim, Andrew |
| Physical access | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@dots.usc.edu |
| Archival file | uscthesesreloadpub_Volume4/etd-KimAndrew-1179.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@dots.usc.edu |
| Full text | HUMAN HISTOLOGICAL ANALYSIS OF AUTOGENOUS BLOCK GRAFTS by Andrew Kim ____________________________________________________ A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (CRANIOFACIAL BIOLOGY) December 2012 Copyright 2012 Andrew Kim |
