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Copyright 2012 Meghann Adrienne Ribbens
SURVIVAL AND EVOLUTION OF SHEWANELLA ONEIDENSIS MR-1:
APPLICATIONS FOR MICROBIAL FUEL CELLS
by
Meghann Adrienne Ribbens
A Dissertation Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
DOCTOR OF PHILOSOPHY
(MOLECULAR BIOLOGY)
August 2012
Object Description
| Title | Survival and evolution of Shewanella oneidensis MR-1: applications for microbial fuel cells |
| Author | Ribbens, Meghann Adrienne |
| Author email | ribbens@usc.edu;maribbens@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Molecular Biology |
| School | College of Letters, Arts And Sciences |
| Date defended/completed | 2012-08-01 |
| Date submitted | 2012-08-01 |
| Date approved | 2012-08-02 |
| Restricted until | 2012-08-02 |
| Date published | 2012-08-02 |
| Advisor (committee chair) | Finkel, Steven E. |
| Advisor (committee member) |
Nealson, Kenneth H. Goodman, Steven D. Berelson, William M. |
| Abstract | Microbial fuel cells are batteries in which microorganisms catalyze the conversion of organic fuel (such as lactate) into protons and electrons that power a resistor (e. g., a light bulb) before reducing the terminal electron acceptor (e. g., oxygen is reduced to water). Great improvements in power production and efficiency have been made by engineering inorganic components, such as the electrodes themselves, to be more efficiently utilized by fuel cell-inhabiting organisms. However, other avenues for improvement may exist, that is, engineering the fuel cell-inhabiting organisms themselves. We hypothesized that Shewanella oneidensis MR-1, a model organism used for studying microbial fuel cells, could be shown to evolve under physiological conditions which mimic those found in microbial fuel cells. These physiological conditions include the planktonic lifestyle, the biofilm lifestyle, and transient association between the two – that is, those cells that rapidly detach from and reattach to the biofilm. ❧ Here we show the Growth Advantage in Stationary Phase (GASP) phenotype conferred by aging cells planktonically in conditions of abundant electron donor and acceptor, as well as conditions of either electron donor or acceptor limitation. In general, the longer cells are aged planktonically, the greater their advantage when competing in a similar environment. A GASP-like phenotype is also conferred by aging cells in a biofilm for 10 days, though aging cells continuously within a biofilm for 20 days resulted in a competitive disadvantage. To better understand cells that are transiently associated with both lifestyles, we observed the rapid formation of, detachment from and reattachment to biofilms. Biofilm spontaneously form both where oxygen is plentiful and where it is scarce. Oxygen-replete biofilms and oxygen-poor biofilms respond to different supplementary amino acids. Response to amino acid supplementation also varies according to the developmental stage of these biofilms. These data may offer insight into the biology of microbial fuel cells, as well as guidance for physiological treatments and methods of directed evolution that will improve microbial fuel cell performance. |
| Keyword | microbial fuel cells; GASP; growth advantage in stationary phase; biofilm; evolution; Shewanella; S. oneidensis MR-1; amino acid |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Contributing entity | University of Southern California |
| Rights | Ribbens, Meghann Adrienne |
| Physical access | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@dots.usc.edu |
| Archival file | uscthesesreloadpub_Volume4/etd-RibbensMeg-1107.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@dots.usc.edu |
| Full text | Copyright 2012 Meghann Adrienne Ribbens SURVIVAL AND EVOLUTION OF SHEWANELLA ONEIDENSIS MR-1: APPLICATIONS FOR MICROBIAL FUEL CELLS by Meghann Adrienne Ribbens A Dissertation Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree DOCTOR OF PHILOSOPHY (MOLECULAR BIOLOGY) August 2012 |
