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THE EFFECT OF LEUCINE-RICH AMELOGENIN PEPTIDE ON
MOUSE BONE DENSITY BY HISTOLOGICAL ANALYSIS
by
Yisu Li
_________________________________________________________
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(CRANIOFACIAL BIOLOGY)
August 2012
Copyright 2012 Yisu Li
Object Description
| Title | The effect of leucine-rich amelogenin peptide on mouse bone density by histological analysis |
| Author | Li, Yisu |
| Author email | yisu.li88@gmail.com;yisuli@usc.edu |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Cranio-Facial Biology |
| School | School of Dentistry |
| Date defended/completed | 2012-06-12 |
| Date submitted | 2012-07-25 |
| Date approved | 2012-07-25 |
| Restricted until | 2012-07-25 |
| Date published | 2012-07-25 |
| Advisor (committee chair) | Paine, Michael |
| Advisor (committee member) |
Oldak, Janet Zhou, Yan Snead, Malcolm |
| Abstract | Objective: The objective of this study was to evaluate the effect of Leucine-rich amelogenin peptide (LRAP) on osteogenesis in the mouse femur model. ❧ Study Design: In a “gain-of-function” study, LRAP was overexpressed in the bone marrow of transgenic mice. In a “loss-of-function” study, an engineered amelogenin cDNA was “knocked in” to the amelogenin locus, resulting in the lack of LRAP expression. Femora were taken from 12-week-old mice and 4 groups of comparisons were made to determine whether there are trabecular bone density differences. In the “gain-of-function” study: male LRAP transgenic mice were compared with male wild-type mice; female LRAP transgenic mice were compared with female wild-type mice. In the “loss-of-function” study: male delta-A hemizygous mice were compared with male wild-type mice; female delta-A homozygous mice were compared with female delta-A heterozygous mice. ❧ Results: No statistically significant bone density difference between the male LRAP transgenic and male wild-type mice was observed. Bone density of female LRAP transgenic mice was statistically higher than that of female wild-type mice. For both male and female mice, there were no statistically significant differences in bone density between the different delta-A genotypes and their corresponding femora. ❧ Conclusions: The osteogenesis effect of LRAP is more evident in the female LRAP transgenic mice. Male LRAP transgenic mice did not exhibit statistically significant bone mass density difference from their wild-type littermates. For the “loss-of-function” design, no statistically significant bone density difference was found for either comparison. Increased sample size is needed and micro-CT analysis is recommended for complete analysis of osteogenesis role of LRAP. |
| Keyword | leucine-rich amelogenin peptide; bone density |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Rights | Li, Yisu |
| Access conditions | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@usc.edu |
| Archival file | uscthesesreloadpub_Volume4/etd-LiYisu-990.pdf |
Description
| Title | Page 1 |
| Full text | THE EFFECT OF LEUCINE-RICH AMELOGENIN PEPTIDE ON MOUSE BONE DENSITY BY HISTOLOGICAL ANALYSIS by Yisu Li _________________________________________________________ A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (CRANIOFACIAL BIOLOGY) August 2012 Copyright 2012 Yisu Li |
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