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PROTEIN AGGREGATION: CURRENT SCENARIO AND RECENT DEVELOPMENTS
by
Manali Shah
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(PHARMACEUTICAL SCIENCES)
May 2012
Copyright 2012 Manali Shah
Object Description
| Title | Protein aggregation: current scenario and recent developments |
| Author | Shah, Manali |
| Author email | manalish@usc.edu;manalishah9@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Pharmaceutical Sciences |
| School | School of Pharmacy |
| Date defended/completed | 2012-05-15 |
| Date submitted | 2012-05-15 |
| Date approved | 2012-05-16 |
| Restricted until | 2012-05-16 |
| Date published | 2012-05-16 |
| Advisor (committee chair) | Shen, Wei-Chiang |
| Advisor (committee member) |
Okamoto, Curtis Toshio Wang, Clay C.C. |
| Abstract | Aggregation of proteins is the major topic of discussion and debate in the biopharmaceutical industry. Various chemical and physical properties of proteins have been studied and manipulated in order to overcome this major hurdle during biopharmaceutical product formulation. Huge investments are done in order to understand the mechanisms underlying abnormal protein aggregation during the production process as well as in-vivo. Theories explaining this aggregation process are manipulated in the industry in order to attain the protein drug in its highest quality. Further, based on the nature and extent of such aggregates formed it is possible to classify the different types of aggregates. As aggregation is assumed to be an end-product of undesirable events occurring through the molecular cascade, based on such classification one can try to reach to the possible causative factor in the molecular cascade that leads to aggregation. Various detection techniques have been utilized to check the presence of aggregate formation while the protein drug is in its initial processing steps. Such techniques provide a lot of information on physical characteristics and real time detection on how the process of aggregation moves in its pathway. Further, we have discussed the most widely used techniques and strategies to inhibit aggregation at the in-vitro level. Storage of proteins in order to maintain it viable for a sufficiently long interval of time is very important. Techniques on effective storage and environmental conditions that may affect as a causative parameter in initiation of aggregation have been discussed. Taking a step further in this direction, many neurodegenerative disorders have found their origins in the formation of protein aggregates, called amyloids. We have discussed two major neurodegenerative diseases: Alzheimer’s disease and Prion Disease and their pathogenesis. |
| Keyword | protein; aggregation; degenerative diseases; protein aggregates; aggregation detection techniques; protein aggregation diseases;mechanisms of protein aggregation; |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Contributing entity | University of Southern California |
| Rights | Shah, Manali |
| Physical access | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@lib.usc.edu |
| Archival file | uscthesesreloadpub_Volume4/etd-ShahManali-853.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | PROTEIN AGGREGATION: CURRENT SCENARIO AND RECENT DEVELOPMENTS by Manali Shah A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (PHARMACEUTICAL SCIENCES) May 2012 Copyright 2012 Manali Shah |
