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Wnt/β-Catenin/p300 Induced Transcription is Critical for
the Differentiation and Maintenance of Paneth Cells.
by
Jia Wu
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(BIOCHEMISTRY AND MOLECULAR BIOLOGY)
May 2014
Copyright 2014 Jia Wu
Object Description
| Title | Wnt/β-catenin/p300 induced transcription is critical for the differentiation and maintenance of Paneth cells |
| Author | Wu, Jia |
| Author email | wjjiaruo1988@hotmail.com;wjjiaruo1988@hotmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biochemistry and Molecular Biology |
| School | Keck School of Medicine |
| Date defended/completed | 2014-03-18 |
| Date submitted | 2014-04-28 |
| Date approved | 2014-04-29 |
| Restricted until | 2014-04-29 |
| Date published | 2014-04-29 |
| Advisor (committee chair) | Tokes, Zoltan A. |
| Advisor (committee member) |
Kahn, Michael Ouellette, Andre J. |
| Abstract | Our lab's previous studies have demonstrated that Wnt/β‐catenin/p300 transcription initiates cell differentiation, whereas Wnt/β‐catenin/cyclic AMP‐responsive element binding protein‐binding protein (CBP) transcription mediates cell proliferation/maintenance of potency. Recent publications have shown that the Wnt signaling pathway induces Paneth cell maturation and Sox9, a Wnt pathway target gene, is required for Paneth cell differentiation. Paneth cells are terminally differentiated epithelial cells in the small intestinal crypts, which contribute to the mucosal antimicrobial barrier by generating and secreting antimicrobial peptides/proteins including α-defensins through their abundant and large granules. We show that β‐catenin/p300 interaction is required for the expression of Sox9 and thereby Paneth cell lineage commitment. Therefore the β‐catenin/p300 interaction is critical for the Paneth cell differentiation and maintenance, in contrast to the β‐catenin/CBP interaction. Our work shows that the blockage of β‐catenin/p300 interaction dramatically reduces the Paneth cell population, which is associated with impaired barrier function. In this way, luminal bacteria can be disseminated through the circulation to the liver and spleen. |
| Keyword | Wnt; β‐Catenin/p300; Paneth cell |
| Language | English |
| Format (imt) | application/pdf |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Contributing entity | University of Southern California |
| Rights | Wu, Jia |
| Physical access | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@lib.usc.edu |
| Filename | etd-WuJia-2446.pdf |
| Archival file | uscthesesreloadpub_Volume8/etd-WuJia-2446.pdf |
Description
| Title | Page 1 |
| Repository email | cisadmin@lib.usc.edu |
| Full text | Wnt/β-Catenin/p300 Induced Transcription is Critical for the Differentiation and Maintenance of Paneth Cells. by Jia Wu A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (BIOCHEMISTRY AND MOLECULAR BIOLOGY) May 2014 Copyright 2014 Jia Wu |
