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Characterization of transgenic zebrafish lines for studying role of Platelet Derived Growth Factor (PDGF) signaling on coronary vessel formation in regenerating zebrafish heart after cryoinjury
by
Gayatri Nair
A Dissertation Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
Master of Science
(Biochemistry and Molecular Biology)
December 2013
Object Description
| Title | Characterization of transgenic zebrafish lines for studying role of platelet derived growth factor (PDGF) signaling on coronary vessel formation in regenerating zebrafish heart after cryoinjury |
| Author | Nair, Gayatri |
| Author email | gayathri6@gmail.com;gayatrin@usc.edu |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biochemistry and Molecular Biology |
| School | Keck School of Medicine |
| Date defended/completed | 2013-10-22 |
| Date submitted | 2013-11-22 |
| Date approved | 2013-11-22 |
| Restricted until | 2013-11-22 |
| Date published | 2013-11-22 |
| Advisor (committee chair) | Lien, Ching Ling (Ellen) |
| Advisor (committee member) |
Stallcup, Michael R. Tokes, Zoltan A. |
| Abstract | It is known that PDGF-B and PDGFRβ interaction in crucial in blood vessel formation in mammals. PDGF-B is involved in proliferation and recruitment of vSMC (vascular smooth muscle cells) and pericytes to blood vessels. Mutants lacking PDGF-B and PDGFRβ show pericyte loss, capillary dilation and rupture. Not much has been known about role of Pdgfb-Pdgfrβ mediated signaling in zebrafish until recently. Now it has been shown that pdgf is upregulated in regenerating zebrafish heart and Pdgf signaling is necessary for intersegmental vessel formation in zebrafish embryo. Also, with the help of chemical inhibitors blocking Pdgfr signaling, it has been shown that Pdgf signaling plays an important role in coronary vessel formation during regeneration in zebrafish. The aim of this project is to help characterize an improved, cleaner and more specific genetic tool which can overcome the shortcoming of chemical inhibitor to study the role of Pdgfrβ signaling in coronary vessel formation during regeneration in zebrafish heart after cryoinjury. |
| Keyword | cryoinjury; heart; PDGF; zebrafish |
| Language | English |
| Format (imt) | application/pdf |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Contributing entity | University of Southern California |
| Rights | Nair, Gayatri |
| Physical access | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@dots.usc.edu |
| Filename | etd-NairGayatr-2177.pdf |
| Archival file | uscthesesreloadpub_Volume8/etd-NairGayatr-2177.pdf |
Description
| Title | Page 1 |
| Repository email | cisadmin@dots.usc.edu |
| Full text | Characterization of transgenic zebrafish lines for studying role of Platelet Derived Growth Factor (PDGF) signaling on coronary vessel formation in regenerating zebrafish heart after cryoinjury by Gayatri Nair A Dissertation Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree Master of Science (Biochemistry and Molecular Biology) December 2013 |
