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A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING
by
Jie GuJie Gu Jie Gu
A Thesis Presented to theA Thesis Presented to theA Thesis Presented to the A Thesis Presented to the A Thesis Presented to the A Thesis Presented to the A Thesis Presented to theA Thesis Presented to theA Thesis Presented to the
FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL OF ENGINEERINGOF ENGINEERINGOF ENGINEERING OF ENGINEERINGOF ENGINEERINGOF ENGINEERINGOF ENGINEERING OF ENGINEERINGOF ENGINEERING
UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of theIn Partial Fulfillment of the In Partial Fulfillment of theIn Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the
Requirements for the DegreeRequirements for the DegreeRequirements for the Degree Requirements for the Degree Requirements for the Degree Requirements for the DegreeRequirements for the DegreeRequirements for the DegreeRequirements for the DegreeRequirements for the Degree
MASTER OF SCIENCE MASTER OF SCIENCE MASTER OF SCIENCE MASTER OF SCIENCEMASTER OF SCIENCEMASTER OF SCIENCE MASTER OF SCIENCEMASTER OF SCIENCE
(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)
May 2012
Copyright 2012Copyright 2012 Copyright 2012Copyright 2012 Copyright 2012Copyright 2012 Jie GuJie Gu Jie Gu
Object Description
| Title | A robust adult zebrafish cardiomyocyte isolation protocol and its application in calcium transients imaging |
| Author | Gu, Jie |
| Author email | billgujie@gmail.com;billgujie@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biomedical Engineering |
| School | Viterbi School of Engineering |
| Date defended/completed | 2012-05-04 |
| Date submitted | 2012-05-04 |
| Date approved | 2012-05-04 |
| Restricted until | 2012-05-04 |
| Date published | 2012-05-04 |
| Advisor (committee chair) | Hsiai, Tzung |
| Advisor (committee member) |
Shung, Kirk Koping Chow, Robert |
| Abstract | The zebrafish is an emerging vertebral animal as a genetically tractable model for human-related disease studies. They serve as models for angiogenesis, drug screening (Serbedzija, Flynn, & Willett, 2000) and more importantly as an emerging tissue regeneration model in cardiovascular research. Biophysical assessment of ion signaling and gap junctions (Severs et al., 2003) is crucial in understanding the electrical conduction phenotypes of single cardiomyocyte, Most of those research are performed with the rat or mice model. In light of the regenerative capacity, zebrafish heart model is garnering enthusiasm. However, their fragile heart and small size have rendered isolation of individual cardiomyocytes a technical challenge. Furthermore, current literature has rendered inconsistent protocols and low yield in isolation. In this context, a robust isolation protocol was developed to demonstrate the application fluorescent Calcium imaging both as the whole heart and single cell levels. Thus, this methodology will provide a basis to incorporate biophysical techniques such as patch-clamp and voltage sensitive dye imaging. |
| Keyword | zebrafish; calcium transients; cardiomyocyte; isolation; |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Rights | Gu, Jie |
| Access conditions | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@usc.edu |
| Archival file | uscthesesreloadpub_Volume4/etd-GuJie-755-0.pdf |
Description
| Title | Page 1 |
| Full text | A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL A ROBUST ADULT ZEBRAFISH CARDIOMYOCYTE ISOLATION PROTOCOL AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING AND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGINGAND ITS APPLICATION IN CALCIUM TRANSIENTS IMAGING by Jie GuJie Gu Jie Gu A Thesis Presented to theA Thesis Presented to theA Thesis Presented to the A Thesis Presented to the A Thesis Presented to the A Thesis Presented to the A Thesis Presented to theA Thesis Presented to theA Thesis Presented to the FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL FACULTY OF THE USC VITERBI SCHOOL OF ENGINEERINGOF ENGINEERINGOF ENGINEERING OF ENGINEERINGOF ENGINEERINGOF ENGINEERINGOF ENGINEERING OF ENGINEERINGOF ENGINEERING UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of theIn Partial Fulfillment of the In Partial Fulfillment of theIn Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the Requirements for the DegreeRequirements for the DegreeRequirements for the Degree Requirements for the Degree Requirements for the Degree Requirements for the DegreeRequirements for the DegreeRequirements for the DegreeRequirements for the DegreeRequirements for the Degree MASTER OF SCIENCE MASTER OF SCIENCE MASTER OF SCIENCE MASTER OF SCIENCEMASTER OF SCIENCEMASTER OF SCIENCE MASTER OF SCIENCEMASTER OF SCIENCE (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) May 2012 Copyright 2012Copyright 2012 Copyright 2012Copyright 2012 Copyright 2012Copyright 2012 Jie GuJie Gu Jie Gu |
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