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ELECTRICAL ELECTRICAL ELECTRICAL ELECTRICAL ELECTRICAL SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS
CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENONCARDIOVASCULAR PHENOMENON
by
Fei Yu Fei YuFei Yu
A Dissertation Dissertation Presented to thePresented to the Presented to the Presented to the Presented to the Presented to the
FACULTY OF THE USC GRADUATE SCHOOLFACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOLFACULTY OF THE USC GRADUATE SCHOOLFACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the
Requirement for the Degree Requirement for the DegreeRequirement for the Degree Requirement for the DegreeRequirement for the Degree Requirement for the Degree Requirement for the DegreeRequirement for the Degree Requirement for the Degree Requirement for the Degree
DOCTOR DOCTOR OF PHILOSOPHYOF PHILOSOPHY OF PHILOSOPHYOF PHILOSOPHY OF PHILOSOPHY
(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)
May 2013 May 2013May 2013May 2013
Copyright 2013 Copyright 2013Copyright 2013Copyright 2013 Copyright 2013 Copyright 2013Copyright 2013 Fei Yu Fei YuFei Yu
Object Description
| Title | Electrical signals to assess cardiovascular phenomenon |
| Author | Yu, Fei |
| Author email | feiyu@usc.edu;ronaldoyu@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Biomedical Engineering |
| School | Viterbi School of Engineering |
| Date defended/completed | 2013-03-25 |
| Date submitted | 2013-05-14 |
| Date approved | 2013-05-14 |
| Restricted until | 2013-05-14 |
| Date published | 2013-05-14 |
| Advisor (committee chair) | Hsiai, Tzung |
| Advisor (committee member) |
Kim, Eun Sok Shung, Kirk K. Yen, Jesse |
| Abstract | Atherosclerosis, particularly mechanically unstable lesions and resulting acute coronary syndromes remain to be one of the leading causes of death and permanent disabilities in the world. We hereby aim to address this clinical issue by applying electrical signals to assess the cardiovascular phenomenon. In particular, we developed novel electrical approaches to two important issues in early diagnosis and treatment of acute coronary syndrome: 1) detection of unstable plaque in vivo for early medical intervention, and 2) assessment of cardiac conduction and mechanical coupling during heart regeneration in small animal models. ❧ Despite advances in diagnosis and therapy, atherosclerotic cardiovascular disease remains the leading cause of morbidity and mortality in the Western world. Predicting metabolically active atherosclerotic lesions has remained an unmet clinical need. We developed an electrochemical strategy to characterize the inflammatory states of high-risk atherosclerotic plaques. Using the concentric bipolar microelectrodes, we sought to demonstrate distinct Electrochemical Impedance Spectroscopic (EIS) measurements for unstable atherosclerotic plaques that harbored active lipids and inflammatory cells. We demonstrated increased impedance in response to oxidized low density lipoprotein (oxLDL)-laden lesions. Using equivalent circuits to simulate vessel impedance at the electrode-endoluminal tissue interface, we demonstrated specific electric elements to model working and counter electrode interfaces as well as the tissue impedance. We hereby assessed the feasibility of integrating EIS with intravascular ultrasound (IVUS) and shear stress (ISS) to provide a new strategy to assess oxLDL-laden lesions in the fat-fed New Zealand White (NZW) rabbits. By applying electrochemical impedance in conjunction with shear stress and high-frequency ultrasound sensors, we provided a new strategy to identify oxLDL-laden lesions. Our study demonstrated the feasibility of integrating EIS, ISS, and IVUS for a catheter-based approach to assess mechanically unstable plaque. ❧ On the other arm, zebrafish (Danio rerio) is an emerging genetic model for regenerative medicine. In humans, myocardial infarction results in the irreversible loss of cardiomyocytes. Zebrafish hearts fully regenerate after resection or cryoinjury, without either scarring or arrhythmias. To study this cardiac regeneration, we developed microelectrode electrocardiograpm (ECG) platform, and subsequently implantable flexible multi-electrode membrane arrays that measure the epicardial electrocardiogram signals of zebrafish in real-time. The microelectrode electrical signals allowed for a high level of both temporal and spatial resolution. We observed delayed electric repolarization in either the regenerated hearts or scar tissues. Early regenerated cardiomyocytes lacked the conduction phenotypes of the sham fish. The electrical signals were in synchrony with optically measured calcium concentration as well as Doppler Echocardiogram. These microelectrode devices therefore provide a real-time analytical tool for monitoring conduction phenotypes of small vertebral animals with a high temporal and spatial resolution. |
| Keyword | atherosclerosis; unstable plaque; ECG; electrochemical impedance spectroscopy; zebrafish |
| Language | English |
| Format (imt) | application/pdf |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Contributing entity | University of Southern California |
| Rights | Yu, Fei |
| Access conditions | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@usc.edu |
| Filename | etd-YuFei-1691.pdf |
| Archival file | uscthesesreloadpub_Volume7/etd-YuFei-1691.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Full text | ELECTRICAL ELECTRICAL ELECTRICAL ELECTRICAL ELECTRICAL SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS SIGNALS TO ASSESS CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENON CARDIOVASCULAR PHENOMENONCARDIOVASCULAR PHENOMENON by Fei Yu Fei YuFei Yu A Dissertation Dissertation Presented to thePresented to the Presented to the Presented to the Presented to the Presented to the FACULTY OF THE USC GRADUATE SCHOOLFACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOLFACULTY OF THE USC GRADUATE SCHOOLFACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIAUNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the In Partial Fulfillment of the Requirement for the Degree Requirement for the DegreeRequirement for the Degree Requirement for the DegreeRequirement for the Degree Requirement for the Degree Requirement for the DegreeRequirement for the Degree Requirement for the Degree Requirement for the Degree DOCTOR DOCTOR OF PHILOSOPHYOF PHILOSOPHY OF PHILOSOPHYOF PHILOSOPHY OF PHILOSOPHY (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING)(BIOMEDICAL ENGINEERING) (BIOMEDICAL ENGINEERING) May 2013 May 2013May 2013May 2013 Copyright 2013 Copyright 2013Copyright 2013Copyright 2013 Copyright 2013 Copyright 2013Copyright 2013 Fei Yu Fei YuFei Yu |
