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PREPARATION AND CHARACTERIZATION OF Tf-G-CSF FUSION PROTEIN
by
Hsin-Fang Lee
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(PHARMACEUTICAL SCIENCES)
August 2008
Copyright 2008 Hsin-Fang Lee
Object Description
| Title | Preparation and characterization of Tf-G-CSF fusion protein |
| Author | Lee, Hsin-Fang |
| Author email | hsinfanl@usc.edu |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Molecular Pharmacology & Toxicology |
| School | School of Pharmacy |
| Date defended/completed | 2008-06-25 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-07-31 |
| Advisor (committee chair) | Shen, Wei-Chiang |
| Advisor (committee member) |
Wang, Clay Okamoto, Curtis |
| Abstract | In this study, Tf-H42-G-CSF fusion protein was engineered for investigating the effects of H4 helical linker 's on the fusion protein expression and the sequence of transferrin and G-CSF moieties on fusion protein activity. Results from this study indicated that H4 linker not only elevated the expression but also increased the in vitro activity of Tf-G-CSF fusion protein. Tf-H42-G-CSF was also compared with G-CSF-H4(2)-Tf for in vitro TfR binding ability, and in vitro and in vivo activity. The results demonstrated that the change in sequence of transferrin and G-CSF moieties had no effect on the in vitro activity and in vitro TfR binding ability of the fusion protein. However, when administered subcutaneously to BDF1 mice, the fusion protein with transferrin at the N-terminal domain exhibited significantly higher in vivo myelopoietic efficacy than the fusion protein with transferrin at the C-terminal domain. |
| Keyword | transferrin; G-CSF; fusion protein |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m1478 |
| Rights | Lee, Hsin-Fang |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-LeeH-20080731 |
| Archival file | uscthesesreloadpub_Volume29/etd-LeeH-20080731.pdf |
Description
| Title | Page 1 |
| Full text | PREPARATION AND CHARACTERIZATION OF Tf-G-CSF FUSION PROTEIN by Hsin-Fang Lee A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (PHARMACEUTICAL SCIENCES) August 2008 Copyright 2008 Hsin-Fang Lee |
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