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20
REFERENCES
11. Arber, DA. Effect of prolonged formalin fixation on the immunohistochemical
reactivity of breast markers. Appl Immunohsitochem Mol Morphol. 2002 June; 10(2):
183-186.
7. Battifora, H (1991) Assessment of Antigen Damage in Immunohistochemistry.
Anatomic. Pathology 95(5): 669-671.
2. Dapson RW (1993) Fixation for the 1990's: a review of needs and accomplishments.
Biotech Histochem 68:75-82.
8. De Marzo, AM, Fedor, HH, Gage, WR, Rubin MA. Inadequate formalin fixation
decreases reliability of p27 immunohistochemical staining: probing optimal fixation time
using high-density tissue microarrays. Hum Pathol. 2002 July 33(7): 756-60.
9. Eltoum I, Fredenburgh J, Myers RB, et al. Introduction to the theory and practice of
fixation of tissues. J Histotechnol. 2001; 24:173-190.
4. Helander KG (1994) Kinetic studies of formaldehyde binding in tissue Biotech
Histochem 64:177-179.
5. Leong, AS, Gilham, PN (1989) The effects of progressive formaldehyde fixation on
the preservation of tissue antigens. Pathology Oct; 21(4): 266-8.
3. Ramos-Vara JA (2005) Technical Aspects of Immunohistochemistry. Vet Pathol 42:
405-426.
1. Shi S-R, Cote RJ, Taylor CR (1997) Antigen Retrieval Immunohistochemistry: Past,
Present, and Future. J Histochem Cytochem 45: 327-344.
6. Shi S-R, Key ME, Kalra KL (1991) Antigen retrieval in formalin-fixed, paraffin-embedded
tissues: an enhancement method for immunohistochemical staining based on
microwave oven heating of tissue sections. 39:741-748.
10. Shi SR, Liu C, Pootrakul L, Tang L, Young A, Chen R, Cote R, Taylor C. Evaluation
of the Value of Frozen Tissue Section Used as “Gold Standard” for
Immunohistochemistry . Am J Clin Pathol 2008; 129: 358-366.
12. Yoshida H, Fukumura Y, Nishida M, Fujita S, Iizuka T. The immunohistochemical
distribution of vimentin in human temporomandibular joint samples. J Oral
Rehabilitation. 2004; 31: 47-51.
Object Description
| Title | Evaluation of the recommendation of vimentin as an internal control for standardization of immunohistochemistry |
| Author | Tang, Laurie |
| Author email | laurie.tang@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Experimental & Molecular Pathology |
| School | Keck School of Medicine |
| Date defended/completed | 2008-06-17 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-07-30 |
| Advisor (committee chair) | Cote, Richard J. |
| Advisor (committee member) |
Shi, Shan-Rong Taylor, Clive R. |
| Abstract | Immunohistochemistry (IHC) using formalin-fixed, paraffin embedded tissues (FFPE) has been used for many years in clinical diagnoses and retrospective studies due to superior preservation of cell morphology. Standardization of IHC has become an important issue for quantitative IHC, for which there is no optimal standard. This study investigates the possibility of using vimentin or histone H1 as internal controls, using tissue microarrays constructed using both pig and human tissues fixed over varying formalin fixation lengths. IHC both with and without antigen retrieval (AR) analysis was conducted, and it was concluded that neither antibodies were viable for use an internal control due to the lack of variance in staining strength over varying fixation times. It was shown that AR equalizes signal strength over most formalin fixation lengths, thus supporting previous work stating that standardization of IHC may use an optimal AR protocol to equalize IHC intensity. |
| Keyword | vimentin; internal control; immunohistochemistry |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m1439 |
| Contributing entity | University of Southern California |
| Rights | Tang, Laurie |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@dots.usc.edu |
| Filename | etd-Tang-20080730 |
| Archival file | uscthesesreloadpub_Volume23/etd-Tang-20080730.pdf |
Description
| Title | Page 26 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@dots.usc.edu |
| Full text | 20 REFERENCES 11. Arber, DA. Effect of prolonged formalin fixation on the immunohistochemical reactivity of breast markers. Appl Immunohsitochem Mol Morphol. 2002 June; 10(2): 183-186. 7. Battifora, H (1991) Assessment of Antigen Damage in Immunohistochemistry. Anatomic. Pathology 95(5): 669-671. 2. Dapson RW (1993) Fixation for the 1990's: a review of needs and accomplishments. Biotech Histochem 68:75-82. 8. De Marzo, AM, Fedor, HH, Gage, WR, Rubin MA. Inadequate formalin fixation decreases reliability of p27 immunohistochemical staining: probing optimal fixation time using high-density tissue microarrays. Hum Pathol. 2002 July 33(7): 756-60. 9. Eltoum I, Fredenburgh J, Myers RB, et al. Introduction to the theory and practice of fixation of tissues. J Histotechnol. 2001; 24:173-190. 4. Helander KG (1994) Kinetic studies of formaldehyde binding in tissue Biotech Histochem 64:177-179. 5. Leong, AS, Gilham, PN (1989) The effects of progressive formaldehyde fixation on the preservation of tissue antigens. Pathology Oct; 21(4): 266-8. 3. Ramos-Vara JA (2005) Technical Aspects of Immunohistochemistry. Vet Pathol 42: 405-426. 1. Shi S-R, Cote RJ, Taylor CR (1997) Antigen Retrieval Immunohistochemistry: Past, Present, and Future. J Histochem Cytochem 45: 327-344. 6. Shi S-R, Key ME, Kalra KL (1991) Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. 39:741-748. 10. Shi SR, Liu C, Pootrakul L, Tang L, Young A, Chen R, Cote R, Taylor C. Evaluation of the Value of Frozen Tissue Section Used as “Gold Standard” for Immunohistochemistry . Am J Clin Pathol 2008; 129: 358-366. 12. Yoshida H, Fukumura Y, Nishida M, Fujita S, Iizuka T. The immunohistochemical distribution of vimentin in human temporomandibular joint samples. J Oral Rehabilitation. 2004; 31: 47-51. |
