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EVALUATION OF THE RECOMMENDATION OF VIMENTIN AS AN INTERNAL
CONTROL FOR STANDARDIZATION OF IMMUNOHISTOCHEMISTRY
by
Laurie Tang
A Dissertation Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(PATHOLOGY)
August 2008
Copyright 2008 Laurie Tang
Object Description
| Title | Evaluation of the recommendation of vimentin as an internal control for standardization of immunohistochemistry |
| Author | Tang, Laurie |
| Author email | laurie.tang@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Experimental & Molecular Pathology |
| School | Keck School of Medicine |
| Date defended/completed | 2008-06-17 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-07-30 |
| Advisor (committee chair) | Cote, Richard |
| Advisor (committee member) |
Shi, Shan-Rong Taylor, Clive |
| Abstract | Immunohistochemistry (IHC) using formalin-fixed, paraffin embedded tissues (FFPE) has been used for many years in clinical diagnoses and retrospective studies due to superior preservation of cell morphology. Standardization of IHC has become an important issue for quantitative IHC, for which there is no optimal standard. This study investigates the possibility of using vimentin or histone H1 as internal controls, using tissue microarrays constructed using both pig and human tissues fixed over varying formalin fixation lengths. IHC both with and without antigen retrieval (AR) analysis was conducted, and it was concluded that neither antibodies were viable for use an internal control due to the lack of variance in staining strength over varying fixation times. It was shown that AR equalizes signal strength over most formalin fixation lengths, thus supporting previous work stating that standardization of IHC may use an optimal AR protocol to equalize IHC intensity. |
| Keyword | vimentin; internal control; immunohistochemistry |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m1439 |
| Rights | Tang, Laurie |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Tang-20080730 |
| Archival file | uscthesesreloadpub_Volume23/etd-Tang-20080730.pdf |
Description
| Title | Page 1 |
| Full text | EVALUATION OF THE RECOMMENDATION OF VIMENTIN AS AN INTERNAL CONTROL FOR STANDARDIZATION OF IMMUNOHISTOCHEMISTRY by Laurie Tang A Dissertation Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (PATHOLOGY) August 2008 Copyright 2008 Laurie Tang |
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