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REGIONAL LOCALIZATION AND REGULATION OF HEMATOPOIETIC STEM
CELLS IN THE BONE MARROW STEM CELL NICHE
by
Narges Rashidi
A Dissertation Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment for the Degree
DOCTOR OF PHILOSOPHY
(SYSTEMS BIOLOGY AND DISEASE)
December 2011
Copyright 2011 Narges Rashidi
Object Description
| Title | Regional localization and regulation of hematopoietic stem cells in the bone marrow stem cell niche |
| Author | Rashidi, Narges |
| Author email | nrashidi@usc.edu;narges.rashidi@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Systems Biology and Disease |
| School | Keck School of Medicine |
| Date defended/completed | 2011-10-11 |
| Date submitted | 2011-11-14 |
| Date approved | 2011-11-14 |
| Restricted until | 2011-11-14 |
| Date published | 2011-11-14 |
| Advisor (committee chair) | Frenkel Baruch |
| Advisor (committee member) |
Adams, Gregor B. Kobielak Krzysztof |
| Abstract | Adult HSCs reside in the BM in a microenvironment known as the stem cell niche. Many studies have identified key components of the niche required to maintain the HSCs in their primitive state. However, other cellular components and the exact location of the HSC niche are yet to be clearly identified. My studies have demonstrated that the coordinated interaction between the osteoblasts and the osteoclasts is required for maintaining HSCs in their BM niche. This also highlighted a structural organization of the localization of primitive hematopoietic cell subsets in distinct regions of the BM. We further examined this spatial regulation of HSCs in two anatomically distinct sites, flat bone and long bones. Our data shows that while HSCs isolated from these two different bones have identical potential, their maintenance may be regulated by different mechanisms. These data further demonstrate that although flat bones are desirable sites for visualizing HSC in their niche, the data derived from imaging HSCs in these bones may not necessarily correlate with functional analysis data that is mainly derived from cells of the long bones. Therefore, we developed an imaging modality based on SHG nanoporbes that can potentially capture the exact localization of HSCs in their niche and their dynamic nature at single cell resolution within all bones. Understanding all of the interactions between the stem cells and their niche may yield both practical methods for manipulating stem cells to achieve therapeutic outcomes and also define a model for the impact of the microenvironment on stem cell biology. |
| Keyword | hematopoietic stem cell; hematopoietic stem cell niche; osteoclast; second harmonic generating nanoprobes |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Rights | Rashidi, Narges |
| Access conditions | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@usc.edu |
| Archival file | uscthesesreloadpub_Volume71/etd-RashidiNar-401.pdf |
Description
| Title | Page 1 |
| Full text | REGIONAL LOCALIZATION AND REGULATION OF HEMATOPOIETIC STEM CELLS IN THE BONE MARROW STEM CELL NICHE by Narges Rashidi A Dissertation Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment for the Degree DOCTOR OF PHILOSOPHY (SYSTEMS BIOLOGY AND DISEASE) December 2011 Copyright 2011 Narges Rashidi |
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