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STUDIES ON THE EXPRESSION AND FUNCTION OF
THE HUMAN TMEM56 PROTEIN
by
Hsiao-Fan Wei
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(BIOCHEMISTRY AND MOLECULAR BIOLOGY)
August 2011
Copyright 2011 Hsiao-Fan Wei
Object Description
| Title | Studies on the expression and function of the human TMEM56 protein |
| Author | Wei, Hsiao-Fan |
| Author email | sumstarwei@gmail.com;hsiaofaw@usc.edu |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biochemistry and Molecular Biology |
| School | Keck School of Medicine |
| Date defended/completed | 2011-05-12 |
| Date submitted | 2011-06-03 |
| Date approved | 2011-06-03 |
| Restricted until | 2011-06-03 |
| Date published | 2011-06-03 |
| Advisor (committee chair) | Tokes, Zoltan |
| Advisor (committee member) |
Zandi, Ebrahim Kalra, Vijay K. |
| Abstract | Transmembrane 56 (TMEM56) is a 30 KDa protein and it is highly conserved in most species. The protein was identified as an IKKβ-interacting protein by mass spectrometry in Dr. Zandi’s Lab. Initially, abnormal aggregation of TMEM56 was observed, which was determined to be due to the boiling step prior to SDS-PAGE. This procedure precluded the detection of protein by Western blotting. To investigate the function of TMEM56, a FLAG-tagged version of it was expressed in human 293T kidney cells, and interacting proteins were identified by co-immunoprecipitation and mass spectrometry. The predominant TMEM56-interacting proteins are mitochondrial F1 components, ATP synthase α and β subunits. TMEM56 and ATP synthase α subunit were shown to be co-localized in the mitochondria. Expression of FLAG-TMEM56 in 293T cells resulted in an increased ATP synthase activity using isolated mitochondria in vitro. On the other hand, siRNA-mediated knock down of TMEM56 decreased the ATP synthase activity in isolated mitochondria. The data presented in this study indicated that TMEM56 is localized in the mitochondria and may play a role in regulating ATP synthesis by interacting with ATP synthase α, and perhaps, the β subunits of the F1 complex. |
| Keyword | ATP synthease enzyme activity assay; LTQ-ETD; mitochondria; TMEM56; transmembrane protein; western blotting |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Rights | Wei, Hsiao-Fan |
| Access conditions | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@usc.edu |
| Archival file | uscthesesreloadpub_Volume71/etd-WeiHsiaoFa-18.pdf |
Description
| Title | Page 1 |
| Full text | STUDIES ON THE EXPRESSION AND FUNCTION OF THE HUMAN TMEM56 PROTEIN by Hsiao-Fan Wei A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (BIOCHEMISTRY AND MOLECULAR BIOLOGY) August 2011 Copyright 2011 Hsiao-Fan Wei |
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