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EVALUATION OF THE ROLE OF THE BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR TWIST-2 IN LYMPHOCYTE DEVELOPMENT
AND ACTIVATION
by
Timothy A. Guyon
________________________________________________________________________
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(MOLECULAR MICROBIOLOGY & IMMUNOLOGY)
August 2011
Copyright 2011 Timothy A. Guyon
Object Description
| Title | Evaluation of the role of the basic Helix-Loop-Helix transcription factor Twist-2 in lymphocyte development and activation |
| Author | Guyon, Timothy A. |
| Author email | timothy.guyon@gmail.com;guyon@usc.edu |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Molecular Microbiology and Immunology |
| School | Keck School of Medicine |
| Date defended/completed | 2011-05-02 |
| Date submitted | 2011-05-31 |
| Date approved | 2011-05-31 |
| Restricted until | 2011-05-31 |
| Date published | 2011-05-31 |
| Advisor (committee chair) | Chen, Si-Yi |
| Advisor (committee member) |
Kalra, Vijay K. Liang, Chengyu |
| Abstract | Transcription factors belonging to the basic helix-loop-helix (bHLH) family have been shown to regulate a variety of signaling pathway and the development and activity of numerous cell types. The bHLH transcription factor Twist-2 is known to be a potent suppressor of NF-κB signaling in several processes including hematopoiesis. In previous research a Twist-2 conventional knockout mouse was found to have a dramatic phenotype including increased myeloid lineage, inflammatory cytokine increase and death shortly after birth. NF-κB signaling has been shown to be crucial for development and activation of lymphocytes, so we hypothesize that Twist-2 may play a role in lymphocyte development and function. The generation and initial characterization of a novel colony of tissue specific Twist-2 knockout mouse created by breeding Twist-2floxed mice with MMTVcre mice is described here. Twist-2fl/fl; MMTVcre mice display no gross dysmorphism and are fertile. Initial characterization of thymocytes from these mice has been performed using flow cytometry analysis of peripheral blood, thymus, lymph node and spleen. Splenic T cells from these mice demonstrated decreased proliferation in an ex Vivo proliferation assay. Preliminary observations suggest that Twist-2 may influence T cell development or activation. |
| Keyword | Immunology; lymphocytes; T cells; Twist-2; TWIST 2 |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m |
| Rights | Guyon, Timothy A. |
| Access conditions | The author retains rights to his/her dissertation, thesis or other graduate work according to U.S. copyright law. Electronic access is being provided by the USC Libraries in agreement with the author, as the original true and official version of the work, but does not grant the reader permission to use the work if the desired use is covered by copyright. It is the author, as rights holder, who must provide use permission if such use is covered by copyright. The original signature page accompanying the original submission of the work to the USC Libraries is retained by the USC Libraries and a copy of it may be obtained by authorized requesters contacting the repository e-mail address given. |
| Repository name | University of Southern California Digital Library |
| Repository address | USC Digital Library, University of Southern California, University Park Campus MC 7002, 106 University Village, Los Angeles, California 90089-7002, USA |
| Repository email | cisadmin@usc.edu |
| Archival file | uscthesesreloadpub_Volume71/etd-GuyonTimot-9-0.pdf |
Description
| Title | Page 1 |
| Full text | EVALUATION OF THE ROLE OF THE BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR TWIST-2 IN LYMPHOCYTE DEVELOPMENT AND ACTIVATION by Timothy A. Guyon ________________________________________________________________________ A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (MOLECULAR MICROBIOLOGY & IMMUNOLOGY) August 2011 Copyright 2011 Timothy A. Guyon |
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