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| Title | Shewanella spc. 16S rDNA signal attenuation due to UVC, gamma and cryogenic lab conditions |
| Author | Fouch, Brenda L. |
| Author email | brenda.fouch@us.army.mil |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Geological Sciences |
| School | College of Letters, Arts and Sciences |
| Date defended/completed | 2008-04-09 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-04-18 |
| Advisor (committee chair) | Nealson, Ken |
| Advisor (committee member) |
Corsetti, Frank Bottjer, David |
| Abstract | In understanding DNA as a biomarker over geologic time scales, it is important to determine the longevity of the signal under common environmental stress. Shewanella spp of bacteria in aqueous solution were subjucted to lethal doses of UVC radiation, gamma radiation and thermal and cryogenic conditions. The species succumbed to UVC quickly with a post-mortem DNA signal at least 2 orders of magnitude longer than the life of the cell. Cryogenic stress revealed differential death, but no obvious signal degradation and gamma radiation showed some degradation with cell death but more data are needed to determine a half life for the signal. This study indicates a DNA signal could last 0 (thermal conditions) to 4 or more (cryogenic conditions) orders of magnitude beyond cell death depending on the environment and the time of cell death. |
| Keyword | DNA signal longevity; rock record; biomarker; gamma; UVC; cyrogenic |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m1151 |
| Rights | Fouch, Brenda L. |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Fouch-20080418 |
| Archival file | uscthesesreloadpub_Volume17/etd-Fouch-20080418.pdf |
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