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QUANTIFICATION OF INTERVERTEBRAL DISC COLLAGEN CROSS-LINKS USING AN
ENZYMATIC DEGRADATION TECHNIQUE
by
Varun Bhardwaj
A Thesis Presented to the
FACULTY OF THE VITERBI SCHOOL OF ENGINEERING
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(BIOMEDICAL ENGINEERING)
December 2007
Copyright 2007 Varun Bhardwaj
Object Description
| Title | Quantification of intervertebral disc collagen cross-links using an enzymatic degradation technique |
| Author | Bhardwaj, Varun |
| Author email | vbhardwa@usc.edu |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biomedical Engineering |
| School | Viterbi School of Engineering |
| Date defended/completed | 2007-08-30 |
| Date submitted | 2007 |
| Restricted until | Unrestricted |
| Date published | 2007-09-10 |
| Advisor (committee chair) | Hedman, Thomas |
| Advisor (committee member) |
D'Argenio, David Khoo, Michael C.K. |
| Abstract | Low back pain is an affliction that is prevalent in people around the world. In many instances, the source of this condition can be traced back to the intervertebral disc. The intervertebral disc is an avascular tissue interposed between the bodies of adjacent vertebrae that is designed for weight-bearing and strength. Due to wear and tear associated with age or an injury, the annulus fibrosis of the intervertebral disc can undergo degenerative changes that result in compression of spinal nerves or nerve roots. Collagen cross-link augmentation in the disc has been studied in order to restore function of the degenerated disc. It has been shown that cross-link augmentation has resulted in increased stability in the spine. However, the effect of various cross-linking agents on the level and rate of collagen cross-linking in intervertebral disc tissue has been unexplored. In this thesis, collagen cross-linking of treated and untreated disc tissue was quantified by enzymatic degradation. The cross-linking agents studied were genipin, methylglyoxal, and methylglyoxal with sodium perborate. The protease used was collagenase. The amount of collagen cross-linking was quantified based on the amount of collagen solubilization over time, where highly cross-linked samples would solubilize at very slow rates. The results of the experiments showed that cross-linking agents could induce a significantly greater level of cross-linking compared to untreated tissue. Specifically, methylglyoxal with sodium perborate cross-linked intervertebral disc tissue more than genipin or methylglyoxal without sodium perborate. Further studies are needed in order to determine the level of cross-linking needed to optimize spinal stability, the rate of cross-linking induced with different concentrations of cross-linking agent and the relative cytotoxicity of various concentrations of cross-linking agents. |
| Keyword | collagen cross-linking; cross-linking; intervertebral disc; spine; back pain; enzymatic degradation; methylglyoxal; genipin |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m810 |
| Rights | Bhardwaj, Varun |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Bhardwaj-20070910 |
| Archival file | uscthesesreloadpub_Volume23/etd-Bhardwaj-20070910.pdf |
Description
| Title | Page 1 |
| Full text | QUANTIFICATION OF INTERVERTEBRAL DISC COLLAGEN CROSS-LINKS USING AN ENZYMATIC DEGRADATION TECHNIQUE by Varun Bhardwaj A Thesis Presented to the FACULTY OF THE VITERBI SCHOOL OF ENGINEERING UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (BIOMEDICAL ENGINEERING) December 2007 Copyright 2007 Varun Bhardwaj |
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