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OBTAINING INSIGHTS INTO BIOMOLECULAR INTERACTIONS THROUGH SURFACE PLASMON RESONANCE
by
Charlene Sen-Yee Lee
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(BIOCHEMISTRY AND MOLECULAR BIOLOGY)
August 2007
Copyright 2007 Charlene Sen-Yee Lee
Object Description
| Title | Obtaining insights into biomolecular interactions through surface plasmon resonance |
| Author | Lee, Charlene Sen-Yee |
| Author email | charlene.s.lee@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biochemistry & Molecular Biology |
| School | Keck School of Medicine |
| Date defended/completed | 2007-06-26 |
| Date submitted | 2007 |
| Restricted until | Unrestricted |
| Date published | 2007-07-24 |
| Advisor (committee chair) | Laird-Offringa, Ite A. |
| Advisor (committee member) |
Tokes, Zoltan A. Bottini, Nunzio |
| Abstract | Surface plasmon resonance allows us to gain kinetic and equilibrium information of interactions occurring in real-time. Our biosensor instrument, BIACORE, has emerged as a powerful tool to study the mechanisms underlying biomolecular interactions. The binding kinetics of two interactions were investigated: HIV-1 Rev protein interacting with the Rev Response Element RNA and the LYP protein interacting with Csk-SH3. The Rev protein is a RNA-binding protein that binds with high affinity to the Rev Response Element RNA. This interaction is necessary for the survival and replication of HIV. A single nucleotide polymorphism in the PTPN22 gene that encodes the LYP protein is strongly associated with autoimmune diseases. Both interaction studies have implications in the role of human disease and possibly targeting an important step of these interactions can aid in clinical therapy. |
| Keyword | surface plasmon resonance; biomolecular interactions; Biacore; Rev-RRE; Lyp-Csk |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m651 |
| Rights | Lee, Charlene Sen-Yee |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Lee-20070724 |
| Archival file | uscthesesreloadpub_Volume23/etd-Lee-20070724.pdf |
Description
| Title | Page 1 |
| Full text | OBTAINING INSIGHTS INTO BIOMOLECULAR INTERACTIONS THROUGH SURFACE PLASMON RESONANCE by Charlene Sen-Yee Lee A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (BIOCHEMISTRY AND MOLECULAR BIOLOGY) August 2007 Copyright 2007 Charlene Sen-Yee Lee |
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