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IDENTIFICATION OF STRAIN-SPECIFIC DNA FRAGMENTS OF
ACTINOBACILLUS ACTINOMYCETEMCOMITANS BY REPRESENTATIONAL
DIFFERENCE ANALYSIS
by
Johanna Mena
____________________________________________________________________
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(CRANIOFACIAL BIOLOGY)
May 2007
Copyright 2007 Johanna Mena
Object Description
| Title | Identification of strain-specific DNA fragments of actinobacillus actinomycetemcomitans by representational difference analysis |
| Author | Mena, Johanna |
| Author email | mena@usc.edu |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Cranio-Facial Biology |
| School | School of Dentistry |
| Date defended/completed | 2007-03-14 |
| Date submitted | 2007 |
| Restricted until | Unrestricted |
| Date published | 2007-04-23 |
| Advisor (committee chair) | Chen, Casey |
| Advisor (committee member) |
Shuler, Charles Slots, Jorgen Zadeh, Homa |
| Abstract | A genomic subtraction method, the representational difference analysis (RDA), was tested for its use in identifying strain-specific DNA in A. actinomycetemcomitans. Subtraction of strain D7S with strain HK1651 yielded D7S-specific 2.3 kb islet-A and 5.3 kb islet-B. Islet-A contains a 1.5 kb region that is homologous to a region found in the A. actinomycetemcomitans plasmid pVT745. Islet-B contains a 2.1 kb homolog of vgr, a component of a DNA repeat element rhs. The distribution of these islets among A. actinomycetemcomitans strains was further examined by PCR. Islet-A was found in 9 serotype a strain and 2 serotype b strains but missing in 37 strains. Islet-B was found in 1 serotype a strain, 4 serotype d strains and 2 serotype e strains, but missing in 41 strains. |
| Keyword | representational difference analysis; actinobacillus actinomycetemcomitans |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m442 |
| Rights | Mena, Johanna |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Mena-20070423 |
| Archival file | uscthesesreloadpub_Volume51/etd-Mena-20070423.pdf |
Description
| Title | Page 1 |
| Full text | IDENTIFICATION OF STRAIN-SPECIFIC DNA FRAGMENTS OF ACTINOBACILLUS ACTINOMYCETEMCOMITANS BY REPRESENTATIONAL DIFFERENCE ANALYSIS by Johanna Mena ____________________________________________________________________ A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (CRANIOFACIAL BIOLOGY) May 2007 Copyright 2007 Johanna Mena |
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