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ASSESSING THE ROLE OF STRAIN-SPECIFIC DNA
IN THE GROWTH AND BIOFILM FORMATION OF
AGGREGATIBACTER ACTINOMYCETEMCOMITANS
by
Sang (Jason) Jee
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(CRANIOFACIAL BIOLOGY)
May 2011
Copyright 2011 Sang (Jason) Jee
Object Description
| Title | Assessing the role of strain-specific DNA in the growth adn biofilm formation of aggregatibacter actinmycetemcomitans |
| Author | Jee, Sang Hyun "Jason" |
| Author email | sjee@usc.edu; shjee84@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Cranio-Facial Biology |
| School | School of Dentistry |
| Date defended/completed | 2011-03-30 |
| Date submitted | 2011 |
| Restricted until | Unrestricted |
| Date published | 2011-05-04 |
| Advisor (committee chair) | Chen, Casey |
| Advisor (committee member) | Paine, Michael,L. Sameshima, Glenn T. |
| Abstract | Strain-specific genes on genomic islands are often acquired by bacteria via horizontal gene transfer (HGT), which may enhance the fitness of the bacteria. Our laboratory has completed a project to compare the genome content of 14 A. actinomycetemcomitans strains recovered from periodontal health and disease. Numerous genomic islands of unknown functions were identified. Some may be nonfunctional vestiges of HGT-acquired genes that have undergone further mutations, while others may function to enhance the virulence of the strains. This study developed an approach to identify strain-specific genes that are involved in growth and biofilm formation of A. actinomycetemcomitans. Experimental variables that may affect the assay outcomes were evaluated. Different approaches for data analysis were also compared. Novel gene genetic protocols were used to construct mutants for A. actinomycetemcomitans strain D7S-1. These mutants were tested for their doubling time and biofilm formation. Mutants with a deletion of a ~5 Kb genomic island (designated as “285-island”) exhibited faster doubling time than the wildtype D7S-1 or other mutants. Deletion mutants restored with a copy of the 285-island exhibited doubling time similar to that of wildtype strain D7S-1. Growth of strain-specific genes in pH 4.0 was also tested and two groups of the mutants were distinguished based on their ability to grow at pH 4.0. This is the first study that demonstrated a function of any strain-specific genomic island (except serotype-specific gene cluster) of A. actinomycetemcomitans, and validated our approach to screen for functionally important genomic islands among A. actinomycetemcomitans strains. |
| Keyword | peridontal disease; dentistry; growth; biofilmActinomycetemcomitans; doubling time; acidic condition |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m3900 |
| Contributing entity | University of Southern California |
| Rights | Jee, Sang Hyun "Jason" |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@dots.usc.edu |
| Filename | etd-Jee-4154 |
| Archival file | uscthesesreloadpub_Volume32/etd-Jee-4154.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@dots.usc.edu |
| Full text | ASSESSING THE ROLE OF STRAIN-SPECIFIC DNA IN THE GROWTH AND BIOFILM FORMATION OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS by Sang (Jason) Jee A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (CRANIOFACIAL BIOLOGY) May 2011 Copyright 2011 Sang (Jason) Jee |
