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THE POLYDISPERSITY OF HUMAN FOXP3 FRAGMENT WITH ZINC
FINGER, LEUCINE ZIPPER AND FORKHEAD DOMAIN
by
Yang Li
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(BIOCHEMISTRY AND MOLECULAR BIOLOGY)
August 2010
Copyright 2010 Yang Li
Object Description
| Title | The polydispersity of human FOXP3 fragment with zinc finger, leucine zipper and forkhead domain |
| Author | Li, Yang |
| Author email | yli6@usc.edu; leonlee0930@hotmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biochemistry & Molecular Biology |
| School | Keck School of Medicine |
| Date defended/completed | 2010-03-29 |
| Date submitted | 2010 |
| Restricted until | Unrestricted |
| Date published | 2010-08-12 |
| Advisor (committee chair) | Tokes, Zoltan |
| Advisor (committee member) |
Chen, Lin Ulmer, Tobias |
| Abstract | Forkhead Box Protein P3 (FOXP3) is essential for the development and maintenance of regulatory T cells (Treg) via regulating the expression of genes involved in TCR signaling. Studies of the homologue of FOXP3, FOXP2, demonstrated that FOXP3 might function as a dimer. However, few papers report the structure of FOXP3 especially its conformation in the solution. In this study, hFOXP3S containing a zinc-finger, a leucine zipper and a forkhead domain, was present as various oligomers in solution. Light scattering assay suggested that the zinc finger affects the conformation of oligomers and the precipitation of the protein was due to the aggregation of large oligomers. DSS is able to crosslink homodimers of hFOXP3S and forkhead FOXP3, but domain-swapping exchange between hFOXP3S and forkhead FOXP3 is not observed. The polydispersity of hFOXP3 solution brings plenty of information for the advanced structural research of FOXP3. |
| Keyword | forkhead domain; polydispersity; zinc finger; structure |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m3393 |
| Contributing entity | University of Southern California |
| Rights | Li, Yang |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Li-3708 |
| Archival file | uscthesesreloadpub_Volume51/etd-Li-3708.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Full text | THE POLYDISPERSITY OF HUMAN FOXP3 FRAGMENT WITH ZINC FINGER, LEUCINE ZIPPER AND FORKHEAD DOMAIN by Yang Li A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (BIOCHEMISTRY AND MOLECULAR BIOLOGY) August 2010 Copyright 2010 Yang Li |
