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MODULATION OF HOST ANTIGEN PRESENTATION BY
HERPES SIMPLEX VIRUS 1
by
Arpita Kulkarni
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(MOLECULAR MICROBIOLOGY AND IMMUNOLOGY)
December 2010
Copyright 2010 Arpita Kulkarni
Object Description
| Title | Modulation of host antigen presentation by herpes simplex virus 1 |
| Author | Kulkarni, Arpita |
| Author email | arpitaku@usc.edu; arpitak@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Molecular Microbiology & Immunology |
| School | Keck School of Medicine |
| Date submitted | 2010 |
| Restricted until | Unrestricted |
| Date published | 2010-09-21 |
| Advisor (committee chair) | Yuan, Weiming |
| Advisor (committee member) |
Schönthal, Axel Machida, Keigo |
| Abstract | Herpes simplex virus 1 is a contagious human pathogen, which affects about 70% of the world population. After a productive infection phase on the skin/mucous membrane, a latency phase follows in the sensory ganglia, which is life-long. CD8 T cells and NKT cells play vital roles in both acute and latent herpes simplex virus 1 infections. Both these components of cell-mediated immunity are activated by antigen presentation through molecules like MHC-I and CD1d. HSV-1 has evolved to evade immune surveillance through mechanisms like latency and establishment of infection in the neurons. By transient and stable expression in mammalian cells, we found that US3 protein kinase and US3.5, its shorter transcript, significantly down-regulate MHC-I from the cell-surface and cause its degradation in the proteasome. Also, US3K220M is a loss of function mutant of this kinase and is not able to down-regulate MHC-I. These results reveal a new role for viral protein kinases in modulation of host antigen presentation. Further, through co-IP and Western blot analysis, we mapped the interaction of CD1d and HSV-1 glycoprotein B to their extracellular domains. |
| Keyword | microbiology; virology; immunology; molecular biology; biochemistry; herpes simplex virus |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m3462 |
| Rights | Kulkarni, Arpita |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Kulkarni-4083 |
| Archival file | uscthesesreloadpub_Volume44/etd-Kulkarni-4083.pdf |
Description
| Title | Page 1 |
| Full text | MODULATION OF HOST ANTIGEN PRESENTATION BY HERPES SIMPLEX VIRUS 1 by Arpita Kulkarni A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (MOLECULAR MICROBIOLOGY AND IMMUNOLOGY) December 2010 Copyright 2010 Arpita Kulkarni |
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