Page 1 |
Save page Remove page | Previous | 1 of 43 | Next |
|
small (250x250 max)
medium (500x500 max)
Large (1000x1000 max)
Extra Large
large ( > 500x500)
Full Resolution
All (PDF)
|
This page
All
|
CALORIE RESTRICTION REDUCES LIVER STEATOSIS IN LIVER SPECIFIC
Pten DELETED MOUSE MODEL
by
Ajeetha Josephrajan
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(BIOCHEMISTRY AND MOLECULAR BIOLOGY)
August 2010
Copyright 2010 Ajeetha Josephrajan
Object Description
| Title | Calorie restriction reduces liver steatosis in liver specific Pten deleted mouse model |
| Author | Josephrajan, Ajeetha |
| Author email | ajosephr@usc.edu; j.ajeetha@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biochemistry & Molecular Biology |
| School | Keck School of Medicine |
| Date defended/completed | 2010-06-22 |
| Date submitted | 2010 |
| Restricted until | Unrestricted |
| Date published | 2010-08-10 |
| Advisor (committee chair) | Tokes, Zoltan A. |
| Advisor (committee member) |
Stiles, Bangyan L. Ulmer, Tobias |
| Abstract | Calorie restriction (CR) is an approach wherein there is moderate restriction of food intake while providing sufficient amount of essential nutrients. CR is beneficial in decreasing tumor progress, physiological aging and increasing the life span of laboratory animals. Previously our study showed that Pten deletion in liver resulted in fatty liver phenotype due to excess accumulation of lipid in liver. In this study, we tested the hypothesis that CR can reduce the burden of fatty liver using our liver Pten model. We showed that CR blocked the triglyceride accumulation in the liver of the Pten null mice. In this study we also investigated the pathway/mechanism involved in this change in phenotype when mice were under calorie restriction. We observed the expression patterns of genes involved in lipogenesis and fatty acid oxidation. Our analysis showed that CR treatment did not alter lipogenesis although lipogenesis expressing genes were induced by CR treatment in Pten control mice. We showed that induction of β -oxidation pathway is likely the reason for the inhibitory effect of CR on liver steatosis in the Pten model. |
| Keyword | calorie restriction; fatty acid oxidation; liver; MCAD; Pten; steatosis |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m3385 |
| Contributing entity | University of Southern California |
| Rights | Josephrajan, Ajeetha |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@lib.usc.edu |
| Filename | etd-Josephrajan-3987 |
| Archival file | uscthesesreloadpub_Volume14/etd-Josephrajan-3987.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | CALORIE RESTRICTION REDUCES LIVER STEATOSIS IN LIVER SPECIFIC Pten DELETED MOUSE MODEL by Ajeetha Josephrajan A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (BIOCHEMISTRY AND MOLECULAR BIOLOGY) August 2010 Copyright 2010 Ajeetha Josephrajan |
