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Parvimonas micra
ADHESION ON DIFFERENT IMPLANT SURFACES:
AN in vitro PILOT STUDY
by
Ching Hsiu Ketty Chiu
_________________________________________________________
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(CRANIOFACIAL BIOLOGY)
August 2010
Copyright 2010 Ching Hsiu Ketty Chiu
Object Description
| Title | Parvimonas micra adhesion on different implant surfaces: an in vitro pilot study |
| Author | Chiu, Ching Hsiu Ketty |
| Author email | chinghsc@usc.edu; kettychiu1009@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Cranio-Facial Biology |
| School | School of Dentistry |
| Date defended/completed | 2010-06-09 |
| Date submitted | 2010 |
| Restricted until | Unrestricted |
| Date published | 2010-06-23 |
| Advisor (committee chair) | Nowzari, Hessam |
| Advisor (committee member) |
Rich, Sandra Navazesh, Mahvash |
| Abstract | Objectives: Despite the high success rate of implant dentistry, failure of implants due to peri-implantitis occurs and may present difficult treatment decisions. Many bacteria have been associated with peri-implantitis, including P. micra. P. micra is considered to be a putative peri-implantitis bacterium which is often isolated from active peri-implantitis. The aim of this in vitro study is to evaluate the ability of P. micra to adhere to different implant surface textures that are currently on the market. In addition, similar surfaced implants between different manufacturing companies were also compared.; Materials and Methods: Seven types of implants were selected: 3i Nanotite implants, 3i Osseotite implants, Implantium anodized implants, Implantium SLA+ implants (calcium phosphate coating), 3i machined surface implants, Branemark TiUnite implants, Branemark machined implants. All implants were divided into three groups, A, B, and C; each consisted of 21 implants (3 sets of seven different implants). Implants were submerged in bacterial broth in atmospheric conditions for 4 hours (group A) and 24 hours (group B) and also in bacteria and nutrient broth under anaerobic conditions for 4 days (group C). Groups A, B, and C were further divided into 3 subgroups, 1, 2, and 3. Subgroups A1, B1, and C1 were incubated with bacteria only. For subgroup A2, B2, and C2, implants were pre-treated with bovine serum to simulate gingival crevicular fluid before submersion into the bacterial broth. As for subgroup A3, B3, and C3, implants were pre-treated with bovine serum as in subgroup 2, and then irrigated with saline after incubation. All implants were immediately fixed, processed and evaluated under scanning electron microscopy. Images of SEM were photographed and used for calculation of the percentage of P. micra adhesion. Adhesion percentages among different surface characteristics and treatments were compared.; Results: P. micra adhesion was on observed in all implant surfaces. Group B presented with the highest percentage of adhesion. When implants were grouped according to their surface characteristics, the average adhesion percentage of rough surface was 16.2% and machined surface was 12.2%. The acid etched group had a higher average of bacterial adhesion (23.2%) when compared to machined (12.6%) and anodized surfaces (5.7%). The average bacterial adhesion percentage for implants with calcium phosphate was 23.4% which is almost twice as high as the non-calcium phosphate treated implants (11.9%). Similar implant surfaces were compared among different companies and 3i machined implants presented with an average adhesion percentage of 6.7 while Branemark machined implants had 16.5%. Implantium anodized implants presented with 6.7% which was very similar to Branemark TiUnite with 4.7%. Effects of serum were inconsistent in the experiment. Saline rinsing appeared to be effective in decreasing the percentage of P. micra, especially in the machined surfaces. Zero (0%) adhesion was observed on machined surfaces (by both manufacturers) in group A and B after saline rinsing.; Conclusion: P. micra adhered to all implant surfaces in this investigation. Surface treatment such as acid etching and deposition of calcium phosphate appeared to increase percentages of P. micra adhesion. Even though the adhesion of P. micra was also observed on the machined surfaces, it may be cautiously concluded by this study of limited samples that smooth surfaces may be more easily cleansable by rinsing. In addition, this study showed that same surface modification produced by different manufacturers may present with different characteristics and possibly alter their interaction with bacteria. However, due to the in vitro nature of this study and the limited number of samples examined, future experiments will be needed to confirm the results of this study. |
| Keyword | bacterial adhesion; dental implant; implant modifications; Parvimonas micra; titanium surface; water rinsing |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m3150 |
| Rights | Chiu, Ching Hsiu Ketty |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Chiu-3872 |
| Archival file | uscthesesreloadpub_Volume17/etd-Chiu-3872.pdf |
Description
| Title | Page 1 |
| Full text | Parvimonas micra ADHESION ON DIFFERENT IMPLANT SURFACES: AN in vitro PILOT STUDY by Ching Hsiu Ketty Chiu _________________________________________________________ A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (CRANIOFACIAL BIOLOGY) August 2010 Copyright 2010 Ching Hsiu Ketty Chiu |
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