Page 1 |
Save page Remove page | Previous | 1 of 17 | Next |
|
small (250x250 max)
medium (500x500 max)
large ( > 500x500)
Full Resolution
All (PDF)
|
This page
All
Subset |
A NOVEL APPROACH FOR DETECTING HYPERCOAGULABILITY UTILIZING
THROMBOELASTOGRAPHY
by
Richard Har Ko
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(CLINICAL AND BIOMEDICAL INVESTIGATIONS)
May 2010
Copyright 2010 Richard Har Ko
Object Description
| Title | A novel approach for detecting hypercoagulability utilizing thromboelastography |
| Author | Ko, Richard Har |
| Author email | richarhk@usc.edu; rko@chla.usc.edu |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Clinical & Biomedical Investigations |
| School | Keck School of Medicine |
| Date defended/completed | 2010-04-05 |
| Date submitted | 2010 |
| Restricted until | Unrestricted |
| Date published | 2010-05-19 |
| Advisor (committee chair) | Young, Guy |
| Advisor (committee member) |
Azen, Stanley Seeger, Robert |
| Abstract | Background: The thromboelastograph (TEG) is a point-of-care global hemostasis assay that measures clot formation and their physical properties, and is licensed for use in monitoring coagulation during complex surgical procedures, but has recently been investigated to monitor patients with bleeding disorders. Although attempts have been made to use the TEG to detect hypercoagulable states, the results have been inconsistent.; Objectives: To develop novel methods utilizing the TEG which are sensitive to detecting hypercoagulability by demonstrating that baseline TEG parameters can be consistently changed so that they will be sensitive to hypercoagulable conditions.; Patients/Methods: To make the TEG more sensitive to detecting hypercoagulability, several pre-analytic modifications were tested on whole blood from healthy adult volunteers with the goal of prolonging the standard clot initiation and clot propagation times. Methods, which resulted in consistent and reliable desired changes, utilized corn trypsin inhibitor (CTI), a contact pathway inhibitor, and blood samples that were not activated. In order to demonstrate that these methods are sensitive to detecting hypercoagulability, increasing concentrations of recombinant human thrombin were added.; Results: The novel methods were able to consistently and statistically significantly change the baseline TEG parameters of R time, K time, and MA in the desired direction. In addition, the novel methods were able to detect increasing concentrations of thrombin.; Conclusions: We describe novel TEG methods which are sensitive to the presence of increasing concentrations of thrombin in vitro. Further studies are under way to determine if these methods will be sensitive to detecting hypercoagulable states in vivo. |
| Keyword | thromboelastography; hypercoagulability |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m3047 |
| Rights | Ko, Richard Har |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Ko-3572 |
| Archival file | uscthesesreloadpub_Volume26/etd-Ko-3572.pdf |
Description
| Title | Page 1 |
| Full text | A NOVEL APPROACH FOR DETECTING HYPERCOAGULABILITY UTILIZING THROMBOELASTOGRAPHY by Richard Har Ko A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (CLINICAL AND BIOMEDICAL INVESTIGATIONS) May 2010 Copyright 2010 Richard Har Ko |
Comments
Post a Comment for Page 1
