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STUDIES OF LACRIMAL GLAND IMMUNE CELL HOMEOSTASIS IN
POLYMERIC IMMUNOGLOBULIN RECEPTOR (pIgR) KNOCKOUT MICE AND
EVALUATION OF MONOCLONAL DIMERIC IgA PRODUCING RABBIT
HYBRIDOMAS
by
Mubashera Kothawala
A Thesis Presented to the
FACULTY OF THE USC GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(PHARMACEUTICAL SCIENCES)
May 2010
Copyright 2010 Mubashera Kothawala
Object Description
| Title | Studies of lacrimal gland immune cell homeostasis in polymeric immunoglobulin receptor (pIgR) knockout mice and evaluation of monoclonal dimeric IgA producing rabbit hybridomas |
| Author | Kothawala, Mubashera |
| Author email | kothawal@usc.edu; mubasherak@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Pharmacy / Pharmaceutical Sciences |
| School | School of Pharmacy |
| Date defended/completed | 2010-03-24 |
| Date submitted | 2010 |
| Restricted until | Unrestricted |
| Date published | 2010-04-20 |
| Advisor (committee chair) | Hamm-Alvarez, Sarah |
| Advisor (committee member) |
Okamoto, Curtis Mircheff, Austin |
| Abstract | Polymeric immunoglobulin receptor, known as pIgR is a characteristic entity of the transcytotic process, being the receptor most widely studied. Its significance is further enhanced by its important biological function, as it transports antibodies, especially dimeric IgA across the epithelium into secretions playing an essential role in the body’s mucosal immune system. Our interest lies in the eye and the lacrimal gland. Since it is already established knowledge that the tears and ocular mucosa contain dimeric IgA as the predominant antibody, we attempted to study an animal model system lacking in this transporter, the pIgR knockout mouse model, in order to ascertain its importance and influence in the function of the lacrimal gland and tear fluid. The level of infiltrating immune cells was increased in the lacrimal gland when pIgR was knocked out, although the exact nature of the inflammation still needs to be determined. These studies showed that absence of pIgR may have an effect on the ocular immune system homeostasis.; In addition to studies on pIgR, our attention was also focused on obtaining a source of rabbit dimeric IgA of a general subclass which acts as a tool to further study the transcytotic machinery involving pIgR and the various proteins interacting with it during transcytosis in lacrimal gland acinar cells. For this purpose, B-cell hybridoma cell lines were generated commercially and the clones were screened using western blotting to identify the ones appearing positive for dimeric IgA. This method demonstrated for the first time, the ability to clone ocular antibody-producing cells. |
| Keyword | dimeric IgA; pIgR; lacrimal gland; rabbit hybridomas |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m2937 |
| Rights | Kothawala, Mubashera |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Kothawala-3626 |
| Archival file | uscthesesreloadpub_Volume17/etd-Kothawala-3626.pdf |
Description
| Title | Page 1 |
| Full text | STUDIES OF LACRIMAL GLAND IMMUNE CELL HOMEOSTASIS IN POLYMERIC IMMUNOGLOBULIN RECEPTOR (pIgR) KNOCKOUT MICE AND EVALUATION OF MONOCLONAL DIMERIC IgA PRODUCING RABBIT HYBRIDOMAS by Mubashera Kothawala A Thesis Presented to the FACULTY OF THE USC GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (PHARMACEUTICAL SCIENCES) May 2010 Copyright 2010 Mubashera Kothawala |
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