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SILENCING OF EXPRESSION OF THE DRIP-80 GENE CORRELATES WITH
ABERRATIONS IN CALCIUM ION DISTRIBUTION IN NICKEL COMPOUND-AND
3-METHYLCHOLANTHRENE-TRANSFORMED C3H/10T1/2 MOUSE
EMBRYO FIBROBLAST CELL LINES
by
Duy Mai
________________________________________________________________________
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(MOLECULAR MICROBIOLOGY AND IMMUNOLOGY)
December 2009
Copyright 2009 Duy Mai
Object Description
| Title | Silencing of expression of the DRIP-80 gene correlates with aberrations in calcium ion distribution in nickel compound- and 3-methylcholanthrene-transformed C3H/10T1/2 mouse embryo fibroblast cell lines |
| Author | Mai, Duy |
| Author email | duymai@usc.edu; duybears@yahoo.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Molecular Microbiology & Immunology |
| School | Keck School of Medicine |
| Date defended/completed | 2009-09-02 |
| Date submitted | 2009 |
| Restricted until | Unrestricted |
| Date published | 2009-10-15 |
| Advisor (committee chair) | Landolph, Joseph |
| Advisor (committee member) |
Schönthal, Axel Ying, Shao-Yao |
| Abstract | 10T1/2 mouse cells were treated with nickel oxide, nickel monosulfide, and 3-methylcholanthrene to establish transformed cell lines. Non-transformed 10T1/2 cells expressed the vitamin D receptor interacting protein 80 (DRIP-80), while nickel ion-transformed cell lines did not. The DRIP-80 protein is a subunit of the Mediator complex, which regulates Ca+2 ion distribution via regulation of vitamin D responsive genes. Disruption of DRIP-80 gene expression may result in an aberrant distribution of Ca+2 ions. To test this hypothesis, non-transformed 10T1/2 cells and transformed 10T1/2 cell lines were stained with Fluo 3-AM and visualized by confocal microscopy. The distribution of Ca+2 ions in non-transformed 10T1/2 cells was heterogeneous. Nickel compound- and MCA-transformed 10T1/2 cells have consistently less Ca+2 ions in the nucleus than the cytoplasm. These results suggest that disruption of normal transport of Ca+2 ions between nucleus and cytoplasm may contribute to the altered phenotypes in transformed 10T1/2 cell lines. |
| Keyword | DRIP-80; calcium ion distribution; 3-methylcholanthrene; nickel oxide; nickel monosulfide; C3H/10T1/2 mouse embryo fibroblast |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m2669 |
| Rights | Mai, Duy |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Mai-3261 |
| Archival file | uscthesesreloadpub_Volume56/etd-Mai-3261.pdf |
Description
| Title | Page 1 |
| Full text | SILENCING OF EXPRESSION OF THE DRIP-80 GENE CORRELATES WITH ABERRATIONS IN CALCIUM ION DISTRIBUTION IN NICKEL COMPOUND-AND 3-METHYLCHOLANTHRENE-TRANSFORMED C3H/10T1/2 MOUSE EMBRYO FIBROBLAST CELL LINES by Duy Mai ________________________________________________________________________ A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (MOLECULAR MICROBIOLOGY AND IMMUNOLOGY) December 2009 Copyright 2009 Duy Mai |
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