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SNAKE VENOM PROTEINS IDENTIFICATION BY NANO HIGH
PERFORMANCE LIQUID CHROMATOGRAPHY TANDEM MASS
SPECTROMETRY
by
Wan-I Kuo
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(BIOCHEMISTRY AND MOLECULAR BIOLOGY)
December 2009
Copyright 2009 Wan-I Kuo
Object Description
| Title | Snake venom proteins identification by nano high performance liquid chromatography tandem mass spectrometry |
| Author | Kuo, Wan-I |
| Author email | wanikuo@usc.edu; bestwani@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biochemistry & Molecular Biology |
| School | Keck School of Medicine |
| Date defended/completed | 2009-09-04 |
| Date submitted | 2009 |
| Restricted until | Unrestricted |
| Date published | 2009-10-15 |
| Advisor (committee chair) | Tokes, Zoltan |
| Advisor (committee member) |
Zandi, Ebrahim Markland, Francis |
| Abstract | In this study, a modified on-column frit preparation method for fused silica capillaries was used to analyze a venom mixture which contained the milkings from approximately ten snakes of the Agkistrodon contortrix contortrix (southern copperhead) species. One hundred and twenty-three proteins were identified by in-gel digestion coupled with nano-HPLC MS. Among them, twenty proteins matched the references and databases identified as snake venom proteins. In addition, de novo sequencing is applied to analyze the collision-induced dissociation (CID) data generated by in-solution digestion coupled with nano-HPLC MS. Twenty-three proteins matched the databases and references previously identified as snake venom proteins.; Because of insufficient sequences in the database, many peptides and hypothetical proteins could not be matched to the venom proteins. Some venom proteins we identified were found in other species. To build up an intact knowledge base to help toxicant study and clinical treatment, this thesis provided a reference for further protein purification, characterization, and function studies |
| Keyword | Agkistrodon contortrix contortrix; mass spectrometry; proteomics; snake venomics |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m2670 |
| Rights | Kuo, Wan-I |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Kuo-3260 |
| Archival file | uscthesesreloadpub_Volume56/etd-Kuo-3260.pdf |
Description
| Title | Page 1 |
| Full text | SNAKE VENOM PROTEINS IDENTIFICATION BY NANO HIGH PERFORMANCE LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY by Wan-I Kuo A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (BIOCHEMISTRY AND MOLECULAR BIOLOGY) December 2009 Copyright 2009 Wan-I Kuo |
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