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POLR2B AND ITS CONTRIBUTIONS TO CANCER CELL GROWTH
by
George Kohan
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(MOLECULAR AND EXPERIMENTAL PATHOLOGY)
December 2009
Copyright 2009 George Kohan
Object Description
| Title | POLR2B and its contributions to cancer cell growth |
| Author | Kohan, George |
| Author email | Gkohan@usc.edu; Masoud2001@aol.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Experimental & Molecular Pathology |
| School | Keck School of Medicine |
| Date defended/completed | 2009-08-06 |
| Date submitted | 2009 |
| Restricted until | Restricted until 21 Feb. 2010. |
| Date published | 2010-02-21 |
| Advisor (committee chair) | Dubeau, Louis |
| Advisor (committee member) |
Hofman, Florence M. Coetzee, Gerhard A. |
| Abstract | Ovarian cancer incidence is highest in the western world, where it is the leading cause of death from gynecological malignancies. Numerous risk factors for ovarian cancer have been identified, including the substantial risk associated with family history of the disease. Genetic predispositions contribute to familial risk, and at least 10% of ovarian cancer incidence is thought to be attributable to genetic factors. Several of the proteins that are associated with ovarian cancer are Transcription Factors that promote cancer cell growth and tumor progression. POLR2B is the second largest subunit of RNA Polymerase II, which is the polymerase responsible for transcription of mRNA in eukaryotes. POLR2B binds to DNA, helps maintain contact in the active site of RNA Polymerase II between the DNA template and the newly synthesized RNA, and plays a key role in facilitating the formation of a phosphodiester bond between an incoming ribonucleoside triphosphate and the elongating RNA transcript. The studies presented here have shown that POLR2B is upregulated in the NM22B ovarian carcinoma cell line to the point that it exceeds the availability of the other subunits of RNA Polymerase II examined, and that knocking down the expression of this subunit via siRNA results in decreased rates of cell growth. These findings suggest that POLR2B has a role, separate from its functions as a subunit of RNA Pol II, as a Transcription Factor that promotes cancer cell growth and tumor progression. |
| Keyword | POLR2B; ovarian cancer; oncogene; HEY/Chr6; NM22B; RNA polymerase II; ovarian carcinoma; genetics; ovarian cancer genetics |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m2573 |
| Contributing entity | University of Southern California |
| Rights | Kohan, George |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@dots.usc.edu |
| Filename | etd-Kohan-3217 |
| Archival file | uscthesesreloadpub_Volume29/etd-Kohan-3217.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@dots.usc.edu |
| Full text | POLR2B AND ITS CONTRIBUTIONS TO CANCER CELL GROWTH by George Kohan A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (MOLECULAR AND EXPERIMENTAL PATHOLOGY) December 2009 Copyright 2009 George Kohan |
