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INFLAMMATORY MECHANISMS IN LOCALIZED AGGRESSIVE
PERIODONTITIS PATIENTS
by
Mahnaz Zandi
_____________________________________________________________________
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(CRANIOFACIAL BIOLOGY)
August 2009
Copyright 2009 Mahnaz Zandi
Object Description
| Title | Inflammatory mechanisms in localized aggressive periodontitis |
| Author | Zandi, Mahnaz |
| Author email | mzandi@usc.edu; mahzandi2002@yahoo.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Cranio-Facial Biology |
| School | School of Dentistry |
| Date defended/completed | 2009-04-15 |
| Date submitted | 2009 |
| Restricted until | Unrestricted |
| Date published | 2009-06-19 |
| Advisor (committee chair) | Nowzari, Hessam |
| Advisor (committee member) |
Rich, Sandra Navazesh, Mahvash |
| Abstract | Introduction: Localized Aggressive Periodontitis (LAP) has a bacterial etiology, with infection of the gingival tissues by several bacterial pathogens. However, in addition to bacterial etiology, host immune and inflammatory factors are implicated in the pathogenesis and progression of localized aggressive periodontitis. Objective: The objective of this study is to explore underlying mechanisms involved in selective sites of infection, and the subsequent inflammatory process in LAP. Methods and Materials: Ten LAP patients participated in this study (3 male and 7 female), with an age range of from 13- 32 years. Samples were taken from one tooth with the deepest pocket for a test site and one tooth with healthy periodontium for a control site from each individual.; Laboratory Tests: Bacterial culture was conducted to evaluate the fourteen periodontal pathogens. Also Real-Time Polymerase Chain Reaction (RT PCR) was done to analyze the presence of Aggrigatibacter actinomycetemcomitans (A.a.) RNA, Cytomegalovirus (CMV) RNA, Nuclear factor kb (NFKb), Tumor Necrosis Factor alpha (TNFa), and Inter Cellular Adhesion Molecule 1 (ICAM1). Results: No periodontal pathogens were present in the control sites. All the test teeth revealed some periodontal pathogens. A.a. was not present in any test samples. The mean percentages of other pathogens in test teeth were 5.28 for Fusobacterium species, 4.90 for Campilobacter species, 3.93 for T. forsythia, 3.53 for P. intermedia, 2.73 for P. gingivais, 1.40 for Enteric gram negative rod, 0.69 for Beta hemolytic streptococci, 0.38 for D. pneumosintes. With PCR analysis CMV RNA and A.a RNA was not present in any of the test or control samples. Measurement of NFkb and ICAM1 expression by RT- PCR showed there was marked increase in the affected sites when compared with control sites. TNFa was not present in any of the test or control samples. Conclusion: The preliminary findings of this study suggest upregulation of active NFkb and ICAM1 play an important role in LAP. |
| Keyword | Locaized aggressive periodontitis; NFKb; ICAM1; TNFa; CMV |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m2308 |
| Contributing entity | University of Southern California |
| Rights | Zandi, Mahnaz |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@dots.usc.edu |
| Filename | etd-Zandi-2957 |
| Archival file | uscthesesreloadpub_Volume56/etd-Zandi-2957.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@dots.usc.edu |
| Full text | INFLAMMATORY MECHANISMS IN LOCALIZED AGGRESSIVE PERIODONTITIS PATIENTS by Mahnaz Zandi _____________________________________________________________________ A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (CRANIOFACIAL BIOLOGY) August 2009 Copyright 2009 Mahnaz Zandi |
