Page 1 |
Save page Remove page | Previous | 1 of 264 | Next |
|
small (250x250 max)
medium (500x500 max)
large ( > 500x500)
Full Resolution
All (PDF)
|
This page
All
Subset |
INFLUENCE OF HORMONE REPLACEMENT THERAPY WITH MAXIMAL ECCENTRIC EXERCISE ON ESTROGEN RECEPTOR COREGULATORS AND SKELETAL MUSCLE DAMAGE IN POSTMENOPAUSAL WOMEN
by
Christina M. Dieli-Conwright
A Dissertation Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
DOCTOR OF PHILOSOPHY
(BIOKINESIOLOGY)
May 2009
Copyright 2009 Christina M. Dieli-Conwright
Object Description
| Title | Influence of hormone replacement therapy with maximal eccentric exercise on estrogen receptor coregulators and skeletal muscle damage in postmenopausal women |
| Author | Dieli-Conwright, Christina M. |
| Author email | cdieli@usc.edu; cteenad@sbcglobal.net |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Biokinesiology & Physical Therapy |
| School | School of Dentistry |
| Date defended/completed | 2009-02-02 |
| Date submitted | 2009 |
| Restricted until | Unrestricted |
| Date published | 2009-04-09 |
| Advisor (committee chair) | Howell, Sandra |
| Advisor (committee member) |
Schroeder, E. Todd Rice, Judd C. Hawkins, Steven Dominguez, Jesus |
| Abstract | The use of hormone replacement therapy (HRT) is a potential treatment to relieve symptoms of menopause and prevent the onset of disease such as osteoporosis in postmenopausal women. Although estrogen has a possible protective effect against exercise-induced skeletal muscle damage, it is unclear if HRT enhances estrogen receptor (ER) transcriptional activity in skeletal muscle at rest and following resistance exercise. We sought to evaluate changes in the gene expression of ER coregulators (SRC-1 and SMRT) and markers of skeletal muscle damage (CK, LDH, and TNF-α) in postmenopausal women following a high-intensity resistance exercise bout. Fourteen postmenopausal women were divided into two groups: Control, women not using HRT (n=6, 59±4 yr, 63±17 kg), or HRT, women using traditional HRT (n=8, 59±4 yr, 89±24 kg). Both groups performed 10 sets of 10 maximal eccentric repetitions of single-leg extension on the Cybex dynamometer at 60°/s with 20 second rest periods between sets. Muscle biopsies of the vastus lateralis were obtained from the exercised leg at baseline and 4 hours after the exercise bout. Gene expression was determined using RT-PCR for SRC-1, SMRT, and TNF-α. Blood draws were performed at baseline and 3 days post-exercise to measure CK and LDH. Independent t-tests were performed to test group differences (Control vs HRT). A probability level of p≤0.05 was used to determine statistical significance. We observed significantly greater changes in gene expression of SRC-1 and SMRT (p≤0.01) and significantly lower changes in gene expression of TNF-α (p≤0.01) in the HRT group compared to controls following the exercise bout. Gene expression of SRC-1 significantly increased (p≤0.01; 2.4 to 5.2 fold change) and gene expression of SMRT significantly decreased (p≤0.01; -1.3 to -4.3 fold change) following the exercise bout in both groups. CK and LDH levels were significantly greater post-exercise (p≤0.01) in the Control group.; These data suggest a single bout of maximal eccentric exercise significantly enhances ER transcriptional activity with greater response present in postmenopausal women using HRT. Postmenopausal women not using HRT experienced greater muscle damage after exercise indicating a possible protective effect of HRT against exercise-induced skeletal muscle damage. |
| Keyword | skeletal muscle; resistance exercise; estrogen |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m2069 |
| Rights | Dieli-Conwright, Christina M. |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-DieliConwright-2666 |
| Archival file | uscthesesreloadpub_Volume44/etd-DieliConwright-2666.pdf |
Description
| Title | Page 1 |
| Full text | INFLUENCE OF HORMONE REPLACEMENT THERAPY WITH MAXIMAL ECCENTRIC EXERCISE ON ESTROGEN RECEPTOR COREGULATORS AND SKELETAL MUSCLE DAMAGE IN POSTMENOPAUSAL WOMEN by Christina M. Dieli-Conwright A Dissertation Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree DOCTOR OF PHILOSOPHY (BIOKINESIOLOGY) May 2009 Copyright 2009 Christina M. Dieli-Conwright |
Comments
Post a Comment for Page 1
