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THE ROLE OF CAP GENE IN SKELETAL AND CARDIAC MUSCLE
FUNCTION
by
Kamal M Kotak
_______________________________________________________
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF CLINICAL AND BIOMEDICAL INVESTIGATIONS
(TRANSLATIONAL RESEARCH TRACT)
May 2009
Copyright 2009 Kamal M Kotak
Object Description
| Title | The role of cap gene in skeletal and cardiac muscle function |
| Author | Kotak, Kamal M. |
| Author email | kkotak@usc.edu; kkotak@fresno.ucsf.edu |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Clinical & Biomedical Investigations |
| School | Keck School of Medicine |
| Date defended/completed | 2008-12-05 |
| Date submitted | 2009 |
| Restricted until | Unrestricted |
| Date published | 2009-03-05 |
| Advisor (committee chair) | Pashmforoush, Mohammad |
| Advisor (committee member) |
Schechter, Joel E. Mircheff, Austin K. |
| Abstract | Objective: The goal of this study was to evaluate the role of the CAP gene in skeletal and cardiac muscle contraction. Accumulating evidence suggests that the proteins in this family are involved in the organization of both signal transduction, glucose uptake and the cytoskeleton. The deletion of CAP should result in poor glucose uptake and poor skeletal and cardiac muscle function.; Methods: The methods used to evaluate the skeletal muscle function were in vitro experiments testing muscle strength and stress exercise tests. For cardiac muscle, we performed a TAC (thoracic aorta ligation) experiment involving response of heart to Isoproterenol. The stress exercise also tested the heart indirectly. Apart from this, we evaluated electron micrographs of both cardiac and skeletal muscles. We also performed a PET scan and Biodistribution study to evaluate glucose uptake in both types of muscles. We also performed Microarray analysis of samples of cardiac tissue.; Results: In the experiment involving the response of the heart rate to Isoproterenol, it was seen that the CAP KO mice were not able to mount heart rates as high as the WT mice in response to the medication. TEM pictures showed abnormal mitochondria in both types of muscles in both types of animals. The microarray analysis showed increased expression of Myosin heavy chains, Natriuretic peptide precursors and the adrenergic receptors. All the other experiments mentioned above failed to show any difference between the CAP KO and WT mice.; Conclusion: In general the results failed to show any difference in performance or glucose uptake of skeletal or cardiac muscles between CAP KO and WT mice. However, when we look at abnormal mitochondria and abnormalities in response to Isoproterenol along with microarray results suggestive of cardiomyopathy, it is a possibility that these mice might be suffering from a mild form of cardiomyopathy. Future experiments directed at knocking out other proteins in the SoHo group and studying expression of MHC proteins would shed more light on the role of these proteins. |
| Keyword | CAP; SoHo; cbl |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Provenance | Electronically uploaded by the author |
| Type | texts |
| Legacy record ID | usctheses-m2000 |
| Contributing entity | University of Southern California |
| Rights | Kotak, Kamal M. |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@dots.usc.edu |
| Filename | etd-Kotak-2576 |
| Archival file | uscthesesreloadpub_Volume48/etd-Kotak-2576.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@dots.usc.edu |
| Full text | THE ROLE OF CAP GENE IN SKELETAL AND CARDIAC MUSCLE FUNCTION by Kamal M Kotak _______________________________________________________ A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF CLINICAL AND BIOMEDICAL INVESTIGATIONS (TRANSLATIONAL RESEARCH TRACT) May 2009 Copyright 2009 Kamal M Kotak |
