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PRODUCTION AND PURIFICATION OF RECOMBINANT MOUSE
APOLIPOPROTEIN F
by
Melinda Miyuki Hom
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(MOLECULAR PHARMACOLOGY AND TOXICOLOGY)
August 2008
Copyright 2008 Melinda Miyuki Hom
Object Description
| Title | Production and purification of recombinant mouse apolipoprotein F |
| Author | Hom, Melinda Miyuki |
| Author email | mhom@usc.edu |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Molecular Pharmacology & Toxicology |
| School | School of Pharmacy |
| Date defended/completed | 2008-07-10 |
| Date submitted | 2008 |
| Restricted until | Unrestricted |
| Date published | 2008-07-31 |
| Advisor (committee chair) | Hamm-Alvarez, Sarah |
| Advisor (committee member) |
Duncan, Roger Mircheff, Austin |
| Abstract | Apolipoprotein F (apoF) is a 35 kDa protein encoded by a cDNA cloned from the murine lacrimal gland. In the present paper, the murine apoF has been expressed as a recombinant histidine-tagged protein in Escherichia coli and purified from expressing cultures. We report here the unique distinctions of apoF including comparisons of apoF with other apolipoproteins (apoD, apoE, and apoN), as well as why this protein was produced. The data presented here provide evidence that isolated recombinant apoF purified in the experimental conditions described here can be used for functional studies. |
| Keyword | lacrimal gland; lipid; apolipoprotein; apolipoprotein F; NOD mouse; Sjögren s syndrome |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m1424 |
| Rights | Hom, Melinda Miyuki |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Hom-20080731 |
| Archival file | uscthesesreloadpub_Volume40/etd-Hom-20080731.pdf |
Description
| Title | Page 1 |
| Full text | PRODUCTION AND PURIFICATION OF RECOMBINANT MOUSE APOLIPOPROTEIN F by Melinda Miyuki Hom A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (MOLECULAR PHARMACOLOGY AND TOXICOLOGY) August 2008 Copyright 2008 Melinda Miyuki Hom |
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