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INVESTIGATING THE ROLE OF SASH1 GENE LOCATED
ON CHROMOSOME 6 IN OVARIAN CANCER
by
Smita Subramanian
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(BIOCHEMISTRY AND MOLECULAR BIOLOGY)
August 2008
Copyright 2008 Smita Subramanian
Object Description
| Title | Investigating the role of SASH1 gene located on chromosome 6 in ovarian cancer |
| Author | Subramanian, Smita |
| Author email | smitags@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biochemistry & Molecular Biology |
| School | Keck School of Medicine |
| Date defended/completed | 2008-06-25 |
| Date submitted | 2008 |
| Restricted until | Restricted until 30 July 2010. |
| Date published | 2010-07-30 |
| Advisor (committee chair) | Tokes, Zoltan |
| Advisor (committee member) |
Dubeau, Louis Laird-Offringa, Ite |
| Abstract | It is known that there is a strong interplay of various oncogenes and tumor suppressor genes underlying the mechanism of cancer formation and identifying these can be instrumental in devising therapeutics approaches. Ovarian cancer being one of the least understood cancers is also a leading cause of death among women and thus requires a deeper insight into its molecular mechanisms. It has been previously reported that there is loss of a chromosomal segment on Chromosome 6 observed in many cancers including ovarian cancer, giving rise to a strong belief that this region possibly harbors one or more crucial tumor suppressor genes. One such candidate gene found to be implicated in breast and colon cancer is SASH1 and the region found to be the hotspot is between 6q24-25. SASH1, located at 6q24.3, has no known function yet but its role is suggested in signaling pathways. In our study, we attempted to elucidate the role of this gene in ovarian cancer. We created an expression vector for this gene earlier thus over expressing it and also exploited the RNAi technology to knock down the expression of this gene and observe its effect on growth rate, cell cycle and apoptosis, by using real-time PCR, growth curve analysis, propidium iodide staining, western blot methods and apoptosis assays. Contrary to the belief that this gene is a candidate tumor suppressor gene in other cancers, our data suggested that reduced SASH1 expression resulted in decreased growth rates and evident apoptosis. By further analysis of its mechanism of action and in-vivo studies, we should be able to propose that this gene plays a definite role in the progression of human ovarian cancer and should be further explored as a possible therapeutic target of ovarian cancer. |
| Keyword | ovarian cancer genetics; tumor suppressor genes |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m1435 |
| Rights | Subramanian, Smita |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Subramanian-20080730 |
| Archival file | uscthesesreloadpub_Volume44/etd-Subramanian-20080730.pdf |
Description
| Title | Page 1 |
| Full text | INVESTIGATING THE ROLE OF SASH1 GENE LOCATED ON CHROMOSOME 6 IN OVARIAN CANCER by Smita Subramanian A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (BIOCHEMISTRY AND MOLECULAR BIOLOGY) August 2008 Copyright 2008 Smita Subramanian |
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