IMMUNE SIGNATURES OF MURINE SOLID TUMOR MODELS by Chern-Yu Yen A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (BIOCHEMISTRY AND MOLECULAR BIOLOGY) August 2009
Copyright 2009 Chern-Yu Yen
This study characterized the major mechanisms of tumor escape at the tumor site employed by eight frequently studied murine solid tumor models by quantitative analysis of immune-related gene expression and immunohistochemistry procedures. The results of these investigations showed that the tumor models used different suppressor cell populations including Treg: Wehi164, B16, and D2F2; MDSC: RENCA, MAD109 and LLC; both Treg and MDSC: 4T1, and a relative lack of either cell population: C26. MAD109, in contrast to the other tumor models, showed high activation of infiltrated lymphoid cells. D2F2, LLC, and RENCA showed incomplete immune activation, with moderate DC activation and upregulation of co-stimulatory factors except for specific deficits. These data suggest that each of the eight tumor models displayed different activation thresholds or used different suppressor mechanisms to escape host immunity. Based upon these data, immunotherapeutic protocols can be tested to counter the evasive mechanisms seen in each tumor model.