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SENCILTM PROJECT:
DESIGN AND FABRICATION OF A DISPOSABLE, PERCUTANEOUS
SENSOR PLATFORM
by
Kuo-Chih Liao
A Dissertation Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
DOCTOR OF PHILOSPHY
(BIOMEDICAL ENGINEERING)
December 2006
Copyright 2006 Kuo-Chih Liao
Object Description
| Title | Sencil project: design and fabrication of a disposable, percutaneous sensor platform |
| Author | Liao, Kuo-Chih |
| Author email | vincent1228@hotmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Biomedical Engineering (Biomedical Imaging & Telemedicine) |
| School | Viterbi School of Engineering |
| Date defended/completed | 2006-07-24 |
| Date submitted | 2006 |
| Restricted until | Unrestricted |
| Date published | 2006-10-05 |
| Advisor (committee chair) | Loeb, Gerald E. |
| Advisor (committee member) |
Hogen-Esch, Theo Richmond, Frances J. Marcu, Laura |
| Abstract | Sencils(TM) (sensory cilia) are optochemical sensors that are minimally invasive, disposable and easily readable to make frequent measurements of various analytes in vivo over a period of 1-3 months. The platform can be adapted to detect different analytes in interstitial fluids. The first application of Sencil senses interstitial glucose based on measurement of fluorescence resonance energy transfer (FRET) between fluorophores bound to betacyclodextrin and Concanavalin A in a polyethylene glycol (PEG) matrix. A percutaneous optical fiber permits precise, reliable photonic measurement of chemical reactions with the nano-engineered polymer matrix attached to the internal end of the fiber. In vitro experiments demonstrate a rapid and precise relationship between the ratio of the two fluorescent emissions and the concentration of glucose in saline over the physiological range of 0-500mg/dl. Chronic implantation in pigs has demonstrated biocompatibility. Another application of the sensor platform is in the identification of effective chemotherapeutic treatment for solid tumors in individual patients. Sencil will deliver the fluorescent agent(s) locally, and detect apoptotic activity initiated by the anticancer treatment. Asymmetric scrambling of phosphatidylserine on cell membrane is one of the indications. The presence of phosphatidylserine on the external surface of apoptotic cells can be detected by increased fluorescence of tiny aliquots of the dye FM 1-43 infused via a microcapillary accompanying the Sencil optical fiber. Preliminary result indicates the capability of this type of Sencil to detect the staurosporine or taxol-induced apoptosis in Matrigel® fixed MCF-7 human breast cancer cells. |
| Keyword | optical fiber; chemical sensor; fluorescence resonance energy transfer; affinity-binding assay; Quantum dots; glucose; cancer |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m84 |
| Rights | Liao, Kuo-Chih |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Liao-20061005 |
| Archival file | uscthesesreloadpub_Volume23/etd-Liao-20061005.pdf |
Description
| Title | Page 1 |
| Full text | SENCILTM PROJECT: DESIGN AND FABRICATION OF A DISPOSABLE, PERCUTANEOUS SENSOR PLATFORM by Kuo-Chih Liao A Dissertation Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree DOCTOR OF PHILOSPHY (BIOMEDICAL ENGINEERING) December 2006 Copyright 2006 Kuo-Chih Liao |
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