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HYPERSPECTRAL AND MULTISPECTRAL OPTICAL BIOLUMINESCENCE
AND FLUORESCENCE TOMOGRAPHY IN SMALL ANIMAL IMAGING
by
Abhijit J. Chaudhari
A Dissertation Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
DOCTOR OF PHILOSOPHY
(ELECTRICAL ENGINEERING)
May 2007
Copyright 2007 Abhijit J. Chaudhari
Object Description
| Title | Hyperspectral and multispectral optical bioluminescence and fluorescence tomography in small animal imaging |
| Author | Chaudhari, Abhijit J. |
| Author email | achaudhari@gmail.com |
| Degree | Doctor of Philosophy |
| Document type | Dissertation |
| Degree program | Electrical engineering |
| School | Viterbi School of Engineering |
| Date defended/completed | 2007-12-13 |
| Date submitted | 2007 |
| Restricted until | Unrestricted |
| Date published | 2007-01-26 |
| Advisor (committee member) |
Leahy, Richard M. Darvas, Felix Proskurowski, Wlodek |
| Abstract | For bioluminescence and fluorescence imaging studies in small animals, it is important to be able to accurately estimate the 3D distribution of a light-emitting source within the animal volume in order to draw biological inferences about underlying processes. The spectrum of light produced by an emission source that escapes the subject varies with the depth of the source because of the wavelength dependence of the optical properties of tissue. Consequently, multispectral or hyperspectral data acquisition should help in the 3D localization of deep sources. In this thesis, we develop a framework for fully 3D bioluminescence and fluorescent tomographic image acquisition and reconstruction that exploits spectral information. Singular value analysis is used both for data dimensionality reduction and to illustrate the advantage of using hyperspectral rather than monochromatic or achromatic data. Regularized tomographic reconstruction techniques that use analytic or numerical solutions to the diffusion approximation of photon transport through turbid media were implemented and their properties were studied in phantom and animal studies. A fixed arrangement of mirrors was used in conjunction with a CCD camera of a bioluminescence imaging system (Xenogen Corporation's IVIS200) for simultaneous acquisition of multispectral imaging data over most of the surface of the animal. Phantom studies conducted using this system demonstrate our ability to accurately localize deep point-like sources and show that a resolution of 1.5 to 2.2 mm for depths up to 6 mm can be achieved. Phantom and simulation studies carried out using a fluorescence imaging system (Cambridge Research Instrument's MAESTRO) demonstrate that sources up to 6 mm depth can be reconstructed using only a single view. For a mouse atlas geometry, we present a method for reconstructing multiple fluorescent probes simultaneously. We also show an in-vivo study of a mouse with a brain tumor expressing firefly luciferase.; Our results indicate good anatomical localization of the tumor when the 3D bioluminescent image was co-registered with magnetic resonance images. While Optical Bioluminescence Tomography (OBT) and Optical Fluorescence Tomography (OFT) are limited to small animal imaging at this stage, a feasibility study using OFT for breast tumor detection in humans shows the potential use of these methods for clinical in-vivo studies. |
| Keyword | hyperspectral; mutispectral; bioluminescence; fluorescence; tomography; small animal imaging |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m232 |
| Rights | Chaudhari, Abhijit J. |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Chaudhari-20070125a |
| Archival file | uscthesesreloadpub_Volume17/etd-Chaudhari-20070125a.pdf |
Description
| Title | Page 1 |
| Full text | HYPERSPECTRAL AND MULTISPECTRAL OPTICAL BIOLUMINESCENCE AND FLUORESCENCE TOMOGRAPHY IN SMALL ANIMAL IMAGING by Abhijit J. Chaudhari A Dissertation Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree DOCTOR OF PHILOSOPHY (ELECTRICAL ENGINEERING) May 2007 Copyright 2007 Abhijit J. Chaudhari |
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