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CONSTRUCTION OF PLASMID FOR CONSTITUTIVE AND INDUCED
EXPRESSION OF SECRETED ALKALINE PHOSPHATASE IN MAMMALIAN
OVARIAN CARCINOMA CELLS
by
Man-Ho Chu
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(BIOCHEMISTRY AND MOLECULAR BIOLOGY)
May 2007
Copyright 2007 Man-Ho Chu
Object Description
| Title | Construction of plasmids for constitutive and induced expression of secreted alkaline phosphatase in mammalian ovarian carcinoma cells |
| Author | Chu, Man-Ho |
| Author email | chumanho@pacbell.net |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biochemistry & Molecular Biology |
| School | Keck School of Medicine |
| Date defended/completed | 2006-12-13 |
| Restricted until | Unrestricted |
| Advisor (committee chair) | Tokes, Zoltan A. |
| Advisor (committee member) |
Dubeau, Louis Stellwagen, Robert H. |
| Abstract | Galectins are lectins which posscess carbohydrate recognition domains that bind ß-galactosides. Secreted alkaline phosphatase (SEAP) is a secreted form of alkaline phosphatase, commonly used as surrogate marker for tumor burden measurement. When expressing SEAP, galectin-3 is down-regulated in ovarian carcinoma cell line ovcar3. Since galectin-3 is known to confer survival advantages to cancer cells, it would be worthwhile to confirm the effect on different cell lines and to elucidate how SEAP causes the down-regulation of galectin-3. This thesis focuses on the construction of SEAPexpressing mammalian expression vectors that could be used as tools to investigate the mechanisms of galectin-3 regulation by SEAP. Two vectors were constructed, one based on a constitutive promoter and the other on an inducible promoter. Also, the ovarian carcinoma cell line HEY was prepared for use in the inducible system by infection with the ecdysone receptor proteins, and was tested for effective induction. |
| Keyword | galectin-3; SEAP; expression plasmid; inducible; ovarian carcinoma cell lines |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m290 |
| Contributing entity | University of Southern California |
| Rights | Chu, Man-Ho |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | cisadmin@lib.usc.edu |
| Filename | etd-Chu-20070223 |
| Archival file | uscthesesreloadpub_Volume23/etd-Chu-20070223.pdf |
Description
| Title | Page 1 |
| Contributing entity | University of Southern California |
| Repository email | cisadmin@lib.usc.edu |
| Full text | CONSTRUCTION OF PLASMID FOR CONSTITUTIVE AND INDUCED EXPRESSION OF SECRETED ALKALINE PHOSPHATASE IN MAMMALIAN OVARIAN CARCINOMA CELLS by Man-Ho Chu A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (BIOCHEMISTRY AND MOLECULAR BIOLOGY) May 2007 Copyright 2007 Man-Ho Chu |
