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THE EFFECT OF NONOXYNOL-9 ON ENDOMETRIAL EXPRESSION OF
SECRTORY LEUKOCYTE PROTEASE INHIBITOR IN-VIVO AND IN-VITRO
by
Megan Aleka Economidis
____________________________________________________________________
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(CLINICAL AND BIOMEDICAL INVESTIGATIONS)
May 2007
Copyright 2007 Megan Aleka Economidis
Object Description
| Title | The effect of nonoxynol-9 on endometrial expression of secretory leukocyte protease inhibitor in-vivo and in-vitro |
| Author | Economidis, Megan Akela |
| Author email | meganecon@gmail.com |
| Degree | Master of Science |
| Document type | Thesis |
| Degree program | Biostatistics |
| School | Keck School of Medicine |
| Date defended/completed | 2006-08-30 |
| Date submitted | 2007 |
| Restricted until | Restricted until 15 Feb. 2009. |
| Date published | 2009-02-15 |
| Advisor (committee member) |
Mishell, Daniel R., Jr. Azen, Stanley Jain, John K. |
| Abstract | Nonoxynol-9 (N-9), a surfactant microbicide, has been shown to damage vaginal epithelium and increase the risk of HIV infection. Little is known about its effect on the immune function of the upper reproductive tract. The purpose of this study is to determine the effect of N-9 on endometrial expression of secretory leukocyte protease inhibitor (SLPI), a naturally occurring protein with antimicrobial activity, in-vivo and in-vitro. Tissue from endometrial biopsies was examined both before and after vaginal administration of N-9. Tissue explants from pre-treatment endometrial biopsies were also used as experimental models. The explants were incubated with three dosages of N-9 for 6 or 24 hours. All biopsy specimens of endometrial tissue and explants were immunohistochemically stained for SLPI. In-vivo there was no statistically significant difference between pre- and post-N-9 SLPI expression in the endometrium. The expression of SLPI was decreased by exposure to N-9 in explants in a dose-dependent manner. These findings indicate that vaginal administration of N-9 does not alter the SLPI mechanism for protecting the endometrium against infection even though N-9 was shown to have a dose-dependent effect on cultured endometrial explants. |
| Keyword | nonoxynol-9; endometrium; secretory leukocyte protease inhibitor |
| Language | English |
| Part of collection | University of Southern California dissertations and theses |
| Publisher (of the original version) | University of Southern California |
| Place of publication (of the original version) | Los Angeles, California |
| Publisher (of the digital version) | University of Southern California. Libraries |
| Type | texts |
| Legacy record ID | usctheses-m268 |
| Rights | Economidis, Megan Akela |
| Repository name | Libraries, University of Southern California |
| Repository address | Los Angeles, California |
| Repository email | http://www.usc.edu/isd/libraries/services/ask_a_librarian/email/ |
| Filename | etd-Economidis-20070215 |
| Archival file | uscthesesreloadpub_Volume32/etd-Economidis-20070215.pdf |
Description
| Title | Page 1 |
| Full text | THE EFFECT OF NONOXYNOL-9 ON ENDOMETRIAL EXPRESSION OF SECRTORY LEUKOCYTE PROTEASE INHIBITOR IN-VIVO AND IN-VITRO by Megan Aleka Economidis ____________________________________________________________________ A Thesis Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (CLINICAL AND BIOMEDICAL INVESTIGATIONS) May 2007 Copyright 2007 Megan Aleka Economidis |
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